Brożek-Mądry Eliza, Ziuzia-Januszewska Laura, Misztal Oliwier, Burska Zofia, Sosnowska-Turek Ewelina, Sierdziński Janusz
Department of Otorhinolaryngology, National Institute of the Ministry of the Interior and Administration, 02-507 Warsaw, Poland.
Faculty of Chemistry, University of Warsaw, 02-093 Warszaw, Poland.
J Clin Med. 2025 May 11;14(10):3341. doi: 10.3390/jcm14103341.
: The evidence regarding the efficacy of probiotics in chronic rhinosinusitis (CRS) is very limited, prompting the EPOS2020 steering group to advise against their use in CRS treatment. Therefore, further research to evaluate the impact of probiotics on microbial communities is particularly important. This study aimed to assess the influence of probiotic nasal rinses on nasal microbiota profiles in patients with primary CRS, granulomatosis with polyangiitis (GPA), and nasal septal perforation (NSP) using 16S rRNA sequencing. : Thirty-six patients with nasal mucosal diseases, including sixteen with primary CRS, eleven with GPA, and nine with NSP, were randomly assigned to either a study group receiving nasal rinses with probiotics containing and , or a control group using nasal rinses with saline. Metagenomic analysis targeting the V3-V4 hypervariable region of the 16S rRNA gene was performed to characterize bacterial and archaeal populations. : At the genus level, the most abundant co-colonizers included , , and After one month of probiotic rinsing, a decrease in abundance of the genera ( = 0.010), ( = 0.020), ( = 0.027), ( = 0.033), ( = 0.035), ( = 0.049), ( = 0.055), ( = 0.055), and ( = 0.058) was observed. Conversely, the abundance of probiotic species and increased. Moreover, increases in the genera and were observed, although they did not reach statistical significance. : Probiotic nasal rinses may contribute to restoring microbial homeostasis by reducing genera associated with inflammatory dysbiosis in nasal inflammatory diseases, warranting further research on their clinical benefits.
关于益生菌在慢性鼻-鼻窦炎（CRS）中疗效的证据非常有限，这促使EPOS2020指导小组建议不要将其用于CRS治疗。因此，进一步研究以评估益生菌对微生物群落的影响尤为重要。本研究旨在通过16S rRNA测序评估益生菌鼻腔冲洗对原发性CRS、肉芽肿性多血管炎（GPA）和鼻中隔穿孔（NSP）患者鼻腔微生物群谱的影响。36例鼻黏膜疾病患者，包括16例原发性CRS患者、11例GPA患者和9例NSP患者，被随机分为接受含 和 的益生菌鼻腔冲洗的研究组或使用生理盐水鼻腔冲洗的对照组。针对16S rRNA基因的V3-V4高变区进行宏基因组分析，以表征细菌和古菌种群。在属水平上，最丰富的共定植菌包括 、 和 。经过一个月的益生菌冲洗后，观察到 属（ = 0.010）、 属（ = 0.020）、 属（ = 0.027）、 属（ = 0.033）、 属（ = 0.035）、 属（ = 0.049）、 属（ = 0.055）、 属（ = 0.055）和 属（ = 0.058）的丰度降低。相反，益生菌物种 和 的丰度增加。此外，观察到 属和 属增加，尽管未达到统计学意义。益生菌鼻腔冲洗可能通过减少与鼻腔炎症性疾病中炎症性生态失调相关的属来有助于恢复微生物稳态，值得进一步研究其临床益处。
