Jîtcă George, Stoicescu Robert, Májai Erzsébet
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Târgu Mureș, 540139 Târgu Mureș, Romania.
Department of Toxicology and Biopharmacy, Faculty of Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Târgu Mureș, 540139 Târgu Mureș, Romania.
Int J Mol Sci. 2025 May 8;26(10):4491. doi: 10.3390/ijms26104491.
Numerous preclinical and clinical studies indicate that CBD possesses various therapeutic properties, including antipsychotic, analgesic, anticonvulsant, antineoplastic, and antioxidant effects. Recent research has also highlighted its potential anxiolytic effects. This study aimed to evaluate the impact of CBD treatment in a PTSD induction model. To determine CBD's efficacy, behavioral tests assessing anxiety and memory were conducted. Additionally, two oxidative stress markers were measured to explore its antioxidant properties. Forty adult male rats were used for PTSD induction. The procedure involved exposure to predator odor on day 10, followed by a second exposure on day 20. A secondary stressor, consisting of daily cage partner changes, was also applied. The animals were randomized into four groups: two non-stressed and two stressed groups. CBD was administered at 10 mg/kg. Behavioral effects were evaluated using the open field (OF), elevated plus maze (EPM), novel object recognition (NOR), and Morris Water Maze (MWM) tests. Malondialdehyde and the GSH/GSSG ratio were assessed using liquid chromatography. CBD treatment did not significantly alter anxiety-like behavior in the EPM, though a trend toward increased vertical exploration was observed in the OF test. In memory-related assessments, no significant differences were found in the NOR test, while performance in the MWM indicated improved spatial memory, with CBD-treated rats spending more time in the target quadrant. In addition, malondialdehyde levels decreased in the CBD groups. Elevated cortisol levels in the stressed CBD group suggest a potential anxiolytic effect, warranting further research.
大量临床前和临床研究表明，大麻二酚（CBD）具有多种治疗特性，包括抗精神病、镇痛、抗惊厥、抗肿瘤和抗氧化作用。最近的研究还强调了其潜在的抗焦虑作用。本研究旨在评估CBD治疗在创伤后应激障碍（PTSD）诱导模型中的影响。为了确定CBD的疗效，进行了评估焦虑和记忆的行为测试。此外，测量了两种氧化应激标志物以探索其抗氧化特性。四十只成年雄性大鼠用于PTSD诱导。该程序包括在第10天暴露于捕食者气味，然后在第20天再次暴露。还施加了由每日更换笼伴组成的二次应激源。动物被随机分为四组：两个非应激组和两个应激组。CBD以10mg/kg的剂量给药。使用旷场（OF）、高架十字迷宫（EPM）、新物体识别（NOR）和莫里斯水迷宫（MWM）测试评估行为影响。使用液相色谱法评估丙二醛和谷胱甘肽/氧化型谷胱甘肽（GSH/GSSG）比值。CBD治疗在EPM中未显著改变焦虑样行为，尽管在OF测试中观察到垂直探索增加的趋势。在与记忆相关的评估中，NOR测试未发现显著差异，而MWM测试中的表现表明空间记忆得到改善，CBD治疗的大鼠在目标象限花费更多时间。此外，CBD组的丙二醛水平降低。应激CBD组中皮质醇水平升高表明可能具有抗焦虑作用，值得进一步研究。
