Mazzone Antonella, Della Rocca Ylenia, Flamminii Federica, Guarnieri Simone, Bello Dainelys Guadarrama, Nanci Antonio, Trubiani Oriana, Diomede Francesca, Pizzicannella Jacopo
Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, 66100 Chieti, Italy.
Laboratory for the Study of Calcified Tissues and Biomaterials, Department of Stomatology, Faculty of Dental Medicine, Université de Montréal, Montréal, QC H3C3J7, Canada.
Int J Mol Sci. 2025 May 21;26(10):4947. doi: 10.3390/ijms26104947.
Barrier function regulation, angiogenic potential, and immune response modulation are only a few of the many roles of the vascular system that nowadays represent one of the main targets for environmental pollutants, in particular, pesticides. We have used human umbilical vein endothelial cells (HUVECs) as an in vitro model to investigate the effects of pesticides on the activation of the NALP3-CASP1-IL-1β inflammatory pathway using real time PCR (RT-PCR) and immunofluorescence investigations, reactive oxygen species (ROS) generation, and morphological alterations with scanning electron microscopy (SEM) analysis. Our findings offer a comprehensive evaluation of the cellular and molecular damage induced by pesticide exposure and show strong inflammasome activation. They indicate that these chemicals may initiate necroptosis and drive prolonged inflammation in endothelial cells. This study provides crucial insights into how pesticides contribute to endothelial dysfunction, highlighting the need for further investigation into their inflammatory and immune-modulatory effects on vascular health.
屏障功能调节、血管生成潜能和免疫反应调节只是血管系统众多作用中的一部分，而如今血管系统是环境污染物（尤其是农药）的主要靶标之一。我们使用人脐静脉内皮细胞（HUVECs）作为体外模型，通过实时聚合酶链反应（RT-PCR）和免疫荧光检测、活性氧（ROS）生成以及扫描电子显微镜（SEM）分析来研究农药对NALP3-CASP1-IL-1β炎症通路激活的影响。我们的研究结果全面评估了农药暴露诱导的细胞和分子损伤，并显示出强烈的炎性小体激活。结果表明，这些化学物质可能引发坏死性凋亡并在内皮细胞中导致持续性炎症。本研究为农药如何导致内皮功能障碍提供了关键见解，凸显了进一步研究其对血管健康的炎症和免疫调节作用的必要性。
