Carneiro Isabelle, da Silva Wellington Luiz Pontes, de Souza Santos Debora Ribeiro, de Filippis Ivano
National Institute for Quality Control in Health, Oswaldo Cruz Foundation-INCQS/FIOCRUZ, Rio de Janeiro 21040-900, Brazil.
Pathogens. 2025 Apr 23;14(5):403. doi: 10.3390/pathogens14050403.
spp. is a major nosocomial pathogen, particularly affecting immunocompromised patients and infants. It is associated with bacteremia, endocarditis, and co-infections. Methicillin-resistant (MRS) carry the gene, encoding PBP2a, which confers resistance to beta-lactam antibiotics. The aim of this study is to investigate resistance profiles and develop a molecular method to identify nosocomial spp. strains. A total of 64 strains from public hospitals in Rio de Janeiro were analyzed using phenotypic and molecular methods, with 17 classified as MDR. Different melting temperatures (Tm) were obtained through qPCR-HRM analysis, to identify (70.4 °C), (79 °C), (74.1 °C) and (70.5 °C)-positive strains (MRS). The gene was detected in 51 strains, with 22 showing SCCmec type IV. The spread of MRSA and MDR , particularly MDR , is a growing concern. In our study, among 64 Staphylococci strains, only 11 were susceptible to methicillin, showing the continuous emergence of resistant strains. qPCR-HRM is a cost-effective, sensitive and fast method for rapid spp. identification, aiding in nosocomial infection control.
葡萄球菌属是一种主要的医院病原体，尤其影响免疫功能低下的患者和婴儿。它与菌血症、心内膜炎及合并感染有关。耐甲氧西林葡萄球菌（MRS）携带编码PBP2a的基因，该基因赋予对β-内酰胺类抗生素的抗性。本研究的目的是调查耐药谱并开发一种分子方法来鉴定医院内葡萄球菌属菌株。使用表型和分子方法对里约热内卢公立医院的64株菌株进行了分析，其中17株被归类为多重耐药。通过qPCR-HRM分析获得了不同的熔解温度（Tm），以鉴定金黄色葡萄球菌（70.4℃）、表皮葡萄球菌（79℃）、溶血葡萄球菌（74.1℃）和腐生葡萄球菌（70.5℃）阳性菌株（MRS）。在51株菌株中检测到了mecA基因，其中22株显示为IV型SCCmec。耐甲氧西林金黄色葡萄球菌和多重耐药葡萄球菌，尤其是多重耐药表皮葡萄球菌的传播，日益引起人们的关注。在我们的研究中，64株葡萄球菌菌株中只有11株对甲氧西林敏感，这表明耐药菌株不断出现。qPCR-HRM是一种经济高效、灵敏且快速的葡萄球菌属鉴定方法，有助于医院感染控制。
