Wassanarungroj Patcharawalai, Nobthai Panida, Ruekit Sirigade, Srijan Apichai, Sukhchat Prawet, Serichantalergs Oralak, Crawford John M, Swierczewski Brett E, Chaudhury Sidhartha, Lertsethtakarn Paphavee
Department of Bacterial and Parasitic Diseases, Walter Reed Army Institute of Research-Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), 315/6 Rajvithi Rd., Bangkok 10400, Thailand.
Queen Sirikit Naval Hospital, Chonburi 20180, Thailand.
Pathogens. 2025 Apr 24;14(5):406. doi: 10.3390/pathogens14050406.
(), especially methicillin-resistant (MRSA), remains a major public health concern. This study reports the antimicrobial resistance profiles and molecular characteristics of 31 isolated during 2017-2018 from inpatient and outpatient clinical specimens from Queen Sirikit Naval Hospital (QSH) in Chonburi province, Thailand. All isolates were tested for antimicrobial susceptibility. Staphylococcal cassette chromosome (SCC) typing, Panton-Valentine leukocidin () toxin, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and staphylococcal protein A () typing were performed. Twenty-seven isolates were confirmed to be MRSA and exhibited resistance to up to seven antibiotics classes. The main MLST type was SCC type II (51.9%) and ST764 (55.6%). Five types were identified with t045 (55.6%) as the major type. All 31 isolates were grouped into seven types using PFGE with the SCCII-ST764-t045 clone being the most prevalent. Overall, our findings reveal that the isolates in this study differ from previous reports in Thailand, indicating a potential shift in local strains, highlighting the need for ongoing molecular surveillance of multidrug resistance patterns of MRSA in Southeast Asia.
（），尤其是耐甲氧西林（MRSA），仍然是一个主要的公共卫生问题。本研究报告了2017年至2018年期间从泰国春武里府诗丽吉王后海军医院（QSH）的住院和门诊临床标本中分离出的31株（）的抗菌药物耐药谱和分子特征。对所有分离株进行了抗菌药物敏感性测试。进行了葡萄球菌盒式染色体（SCC）分型、杀白细胞素（）毒素分型、脉冲场凝胶电泳（PFGE）、多位点序列分型（MLST）和葡萄球菌蛋白A（）分型。27株分离株被确认为MRSA，对多达7类抗生素耐药。主要的MLST型为SCC II型（51.9%）和ST764（55.6%）。鉴定出5种（）型，其中t045（55.6%）为主要类型。使用PFGE将所有31株（）分离株分为7种类型，其中SCCII-ST764-t045克隆最为常见。总体而言，我们的研究结果表明，本研究中的（）分离株与泰国以前的报告不同，表明当地菌株可能发生了变化，突出了对东南亚MRSA多重耐药模式进行持续分子监测的必要性。
