Lins José Gabriel G, Amarante Alessandro F T
Department of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618-681, Brazil.
Department of Biostatistics and Biodiversity, Institute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-689, Brazil.
Pathogens. 2025 May 1;14(5):447. doi: 10.3390/pathogens14050447.
is a highly pathogenic blood-sucking nematode from the abomasum of small ruminants. To develop effective control strategies, it is essential to understand the initial mechanisms involved in host resistance to this parasite. In this study, we used computational tools to analyze the complement and coagulation pathways generated from RNA sequencing of abomasal tissue from resistant (Santa Ines) and susceptible (Ile de France) young sheep artificially infected with . Thirty-two differentially expressed genes annotated to the ovine genome were associated with the complement and coagulation cascades, of which 29 of them were overexpressed in Santa Ines. Our data identified potential markers for resistance trait selection in sheep, such as C3 (complement C3), F3 (tissue factor), F5 (coagulation factor V), CFB (complement factor B), and CFI (complement factor I). Santa Ines may have a more robust coagulation system, being activated by extrinsic pathways associated with tissue damage. The complement may act as a mediator of the innate immunity, and its activation in Santa Ines is associated with the classical, the lectin, and the alternative pathway. Finally, resistant Santa Ines lambs had a polygenic overexpressed architecture controlling both complement and coagulation cascades, which probably contributed to the early-onset protection against .
是一种来自小反刍动物皱胃的高致病性吸血线虫。为制定有效的控制策略，了解宿主对这种寄生虫的抗性初始机制至关重要。在本研究中，我们使用计算工具分析了人工感染的抗性（圣伊内斯）和易感（法兰西岛）幼羊皱胃组织RNA测序产生的补体和凝血途径。注释到绵羊基因组的32个差异表达基因与补体和凝血级联相关，其中29个在圣伊内斯中过度表达。我们的数据确定了绵羊抗性性状选择的潜在标志物，如C3（补体C3）、F3（组织因子）、F5（凝血因子V）、CFB（补体因子B）和CFI（补体因子I）。圣伊内斯可能具有更强健的凝血系统，由与组织损伤相关的外源性途径激活。补体可能作为先天免疫的介质，其在圣伊内斯中的激活与经典途径、凝集素途径和替代途径相关。最后，抗性圣伊内斯羔羊具有控制补体和凝血级联的多基因过度表达结构，这可能有助于对的早期保护。
