Tamaru Masae, Sakaguchi Seiho, Souzu Yasuhiro, Murata Koichi, Yunus Muchammad, Rosyadi Imron, Sato Hiroshi
Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
Department of Parasitology, Faculty of Veterinary Medicine, Airlangga University, Mulyorejo, Surabaya 60115, Indonesia.
Pathogens. 2025 May 6;14(5):455. doi: 10.3390/pathogens14050455.
Fine nematodes of the family Capillariidae parasitize various organs and tissues in fish, amphibians, reptiles, avians, and mammals. Currently classified into more than 20 genera, these nematodes are primarily distinguished based on the caudal structures of male worms. Morphological and molecular analyses were conducted on 15 mammal-parasitic species belonging to the genera (, n. sp., n. comb. (syn. ), , and ), ( n. sp., , n. sp., and n. sp.), (), (), ( n. sp.), and ( n. sp., , and sp.), using specimens from various wild and domestic animals in Japan and brown rats in Indonesia. As demonstrated in this study, nearly complete SSU rDNA sequencing is a powerful tool for differentiating closely related species and clarifying the phylogenetic relationships among morphologically similar capillariid worms. Additionally, most capillariid worms detected in dogs and cats are suspected to be shared with their respective wildlife reservoir mammals. Therefore, molecular characterization, combined with the microscopic observation of these parasites in wildlife mammals, provides a robust framework for accurate species identification, reliable classification, and epidemiological assessment.
毛细科的细线虫寄生于鱼类、两栖类、爬行类、鸟类和哺乳类的各种器官和组织中。这些线虫目前被分为20多个属，主要根据雄虫的尾部结构来区分。利用日本各种野生动物和家畜以及印度尼西亚褐家鼠的标本，对属于（属名）、（新种名）、（新组合名，同义词）、（属名）、（属名）、（新种名）、（属名）、（属名）、（新种名）、（新种名）、（种名）和（新种名）、（种名）、（种名）的15种寄生于哺乳动物的物种进行了形态学和分子分析。如本研究所示，近乎完整的小亚基核糖体DNA测序是区分近缘物种以及阐明形态相似的毛细科线虫之间系统发育关系的有力工具。此外，在犬类和猫类中检测到的大多数毛细科线虫被怀疑与其各自的野生动物宿主哺乳动物共有。因此，分子特征分析与对野生动物哺乳动物体内这些寄生虫的显微镜观察相结合，为准确的物种鉴定、可靠的分类和流行病学评估提供了一个强大的框架。
