Sun Xiaoyan, Li Yan, Wang Haifeng, Meng Yanqiu, Dai Xu, Du Lina, Li Lei
College of Chemical Engineering, Shenyang University of Chemical Technology, Shenyang 110142, China.
Pharmaceutics. 2025 May 13;17(5):644. doi: 10.3390/pharmaceutics17050644.
A sustainable inflammatory response is a significant obstacle for diabetic wound care. In this study, the pH-sensitive multifunctional hydrogel ODex/BSA-Zn was fabricated via a Schiff base and coordination force for the first time. The hydrogel consisted of oxidized dextran (ODex), bovine serum albumin (BSA), and zinc ions (Zn) in the absence of an additional crosslinking agent. The hydrogel showed excellent mechanical stability, fast self-healing ability, and significant anti-inflammatory effects, as demonstrated by the formation of dynamic covalent bonds between the aldehyde group (-CHO) of ODex and the amino group (-NH) of BSA via the Schiff base reaction, as well as the metal-ion coordination reaction of Zn with the imidazole ring of BSA. In a diabetic mouse full-thickness cutaneous defect wound model, the ODex/BSA-Zn hydrogel could effectively inhibit the inflammatory response and increase collagen deposition, thereby accelerating the transition of macrophage M1 to M2 and promoting wound closure. This study offers a promising therapeutic approach for managing long-term diabetic ulcers.
持续的炎症反应是糖尿病伤口护理的一个重大障碍。在本研究中，首次通过席夫碱和配位作用制备了pH敏感的多功能水凝胶ODex/BSA-Zn。该水凝胶由氧化葡聚糖（ODex）、牛血清白蛋白（BSA）和锌离子（Zn）组成，无需额外的交联剂。通过ODex的醛基（-CHO）与BSA的氨基（-NH）之间通过席夫碱反应形成动态共价键，以及Zn与BSA的咪唑环的金属离子配位反应，证明该水凝胶具有优异的机械稳定性、快速自愈能力和显著的抗炎作用。在糖尿病小鼠全层皮肤缺损伤口模型中，ODex/BSA-Zn水凝胶可有效抑制炎症反应并增加胶原蛋白沉积，从而加速巨噬细胞M1向M2的转变并促进伤口愈合。本研究为长期糖尿病溃疡的治疗提供了一种有前景的治疗方法。
