从乳制品中分离和鉴定产β-苯乙胺的乳酸菌

Isolation and Characterization of β-Phenylethylamine-Producing Lactic Acid Bacteria from Dairy Products.

作者信息

Casado Angel, Fernández Eva, González Héctor, Fernández María, Alvarez Miguel A, Ladero Victor

机构信息

Department of Technology and Biotechnology of Dairy Products, Dairy Research Institute, IPLA-CSIC, C/Francisco Pintado Fe 26, 33011 Oviedo, Asturias, Spain.

Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Av. del Hospital Universitario s/n, 33011 Oviedo, Asturias, Spain.

出版信息

Microorganisms. 2025 Apr 23;13(5):966. doi: 10.3390/microorganisms13050966.

Abstract

β-phenylethylamine (PEA) is a neuroactive trace amine synthesized by the enzymatic decarboxylation of phenylalanine. PEA is involved in the improvement of mood and attention. Functional foods enriched in this compound could, therefore, be of interest to the food industry. PEA is produced by microbial activity in certain foods, but usually only in small amounts. The search for PEA-producing microorganisms with good technological properties is thus a pre-requisite if such functional foods are to be produced. This work reports the isolation of thirty-three PEA-producing bacterial strains from samples of different dairy products. They belong to the genus , and the species . Identified strains of were then selected for technological characterization. Some of them showed properties of interest. In this species, PEA was determined to be produced via the action of tyrosine decarboxylase, encoded by the gene This implies that, apart from PEA, a concomitant production of tyramine, a toxic biogenic amine, was observed.

摘要

β-苯乙胺(PEA)是一种通过苯丙氨酸的酶促脱羧作用合成的具有神经活性的痕量胺。PEA 参与情绪和注意力的改善。因此,富含这种化合物的功能性食品可能会引起食品行业的兴趣。PEA 在某些食品中由微生物活动产生,但通常产量很少。因此,如果要生产此类功能性食品,寻找具有良好工艺特性的产 PEA 微生物是一个先决条件。这项工作报道了从不同乳制品样品中分离出 33 株产 PEA 的细菌菌株。它们属于 属和 种。然后选择已鉴定的 菌株进行工艺特性分析。其中一些显示出令人感兴趣的特性。在这个物种中,PEA 被确定是通过由基因 编码的酪氨酸脱羧酶的作用产生的。这意味着,除了 PEA 之外,还观察到了有毒生物胺酪胺的伴随产生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/181f/12114284/b3ce80df5206/microorganisms-13-00966-g001.jpg

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