使用蓝光荧光显微镜诊断分枝杆菌病时的涂片结果。
Smear results in the diagnosis of mycobacterioses using blue light fluorescence microscopy.
作者信息
Pollock H M, Wieman E J
出版信息
J Clin Microbiol. 1977 Mar;5(3):329-31. doi: 10.1128/jcm.5.3.329-331.1977.
Abstract
Examination of 6,880 sputum specimens from untreated patients disclosed that 3.1% were positive for mycobacteria by fluorescence microscopy, and 92.5% of these has positive cultures. There was a positive correlation between the number of organisms seen on smear and growth on culture. All specimens with positive smears and negative cultures contained rare or few acid-fast bacilli on the smear. Eighty-two percent of the specimens with positive cultures and negative smears yielded less than 25 colonies, whereas there were greater than 25 colonies from 93% of the specimens with positive smears and cultures. In a low prevalence, general hospital population, the primary acid-fast smear continued to be a reliable diagnostic tool.
摘要
对6880份来自未经治疗患者的痰液标本进行检查发现，通过荧光显微镜检查，3.1%的标本结核分枝杆菌呈阳性，其中92.5%培养结果为阳性。涂片上可见的菌量与培养生长之间存在正相关。所有涂片阳性但培养阴性的标本涂片上含有罕见或少量抗酸杆菌。培养阳性但涂片阴性的标本中，82%培养出的菌落数少于25个，而涂片和培养均阳性的标本中93%培养出的菌落数大于25个。在患病率较低的综合医院人群中，初次抗酸涂片仍然是一种可靠的诊断工具。
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