Jiang Tao, Wang Chengli, Zhang Mian, Qiao Yongli, Kang Zhensheng, Huang Lili
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China.
Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, China.
Mol Plant Pathol. 2025 May;26(5):e70097. doi: 10.1111/mpp.70097.
The apple Valsa canker caused by Valsa mali is one of the most destructive trunk diseases in apple production and disease management. Understanding the interaction between the pathogen and host is a critical foundation for developing durable disease control technologies. In this study, we showed that VmSR1 from V. mali can suppress the plant immune response and promote pathogen infection. VmSR1 associates with the Tudor staphylococcal nuclease 2 (TSN2) proteins in Malus domestica (apple), Arabidopsis thaliana and Nicotiana benthamiana, promotes degradation of TSN2 proteins, and suppresses the abundance of multiple miRNAs an Silencing of TSN2 significantly reduced the abundance of miRNAs and weakened the resistance of apple leaves to V. mali as well as N. benthamiana to Sclerotinia sclerotiorum. These findings expand the understanding of the function of effectors as RNA silencing suppressors during host-pathogen interactions and deepen the understanding of effectors regulating host immunity.
由苹果黑腐皮壳菌引起的苹果腐烂病是苹果生产和病害管理中最具毁灭性的枝干病害之一。了解病原体与宿主之间的相互作用是开发持久病害控制技术的关键基础。在本研究中，我们表明来自苹果黑腐皮壳菌的VmSR1可以抑制植物免疫反应并促进病原体感染。VmSR1在苹果、拟南芥和本氏烟草中与Tudor葡萄球菌核酸酶2（TSN2）蛋白相互作用，促进TSN2蛋白的降解，并抑制多种miRNA的丰度。TSN2的沉默显著降低了miRNA的丰度，并削弱了苹果叶片对苹果黑腐皮壳菌的抗性以及本氏烟草对核盘菌的抗性。这些发现扩展了对效应子在宿主-病原体相互作用中作为RNA沉默抑制子功能的理解，并加深了对效应子调节宿主免疫的理解。
