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在人类精子中发现一种含γ-羧基谷氨酸的蛋白质。

Discovery of a gamma-carboxyglutamic acid-containing protein in human spermatozoa.

作者信息

Soute B A, Müller-Esterl W, de Boer-van den Berg M A, Ulrich M, Vermeer C

出版信息

FEBS Lett. 1985 Oct 7;190(1):137-41. doi: 10.1016/0014-5793(85)80444-0.

DOI:10.1016/0014-5793(85)80444-0
PMID:4043391
Abstract

Here we describe the identification of a gamma-carboxyglutamic acid-containing protein in human spermatozoa. After thermal decarboxylation the protein is a good substrate for vitamin K-dependent carboxylase from various origins. A quick purification procedure for the decarboxylated protein is presented and in a preliminary characterization we have established its Mr (28 000-30 000) and its amino acid composition.

摘要

在此,我们描述了在人类精子中鉴定出一种含γ-羧基谷氨酸的蛋白质。热脱羧后,该蛋白质是来自各种来源的维生素K依赖性羧化酶的良好底物。本文介绍了一种对脱羧蛋白的快速纯化方法,并且在初步表征中,我们确定了其分子量(28000 - 30000)及其氨基酸组成。

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1
Discovery of a gamma-carboxyglutamic acid-containing protein in human spermatozoa.在人类精子中发现一种含γ-羧基谷氨酸的蛋白质。
FEBS Lett. 1985 Oct 7;190(1):137-41. doi: 10.1016/0014-5793(85)80444-0.
2
A method for decarboxylation of gamma-carboxyglutamic acid in proteins. Properties of the decarboxylated gamma-carboxyglutamic acid protein from calf bone.一种蛋白质中γ-羧基谷氨酸脱羧的方法。来自小牛骨的脱羧γ-羧基谷氨酸蛋白的性质。
J Biol Chem. 1979 Jan 25;254(2):431-6.
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Quantitative analysis and comparative decarboxylation of aminomalonic acid, beta-carboxyaspartic acid, and gamma-carboxyglutamic acid.氨基丙二酸、β-羧基天冬氨酸和γ-羧基谷氨酸的定量分析及比较脱羧作用
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Analysis of gamma-carboxyglutamic acid by gas-liquid chromatography.用气液色谱法分析γ-羧基谷氨酸
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引用本文的文献

1
Direct measurement of vitamin K-dependent enzymes in various isolated and cultured tumor and non-tumor cells.在各种分离和培养的肿瘤及非肿瘤细胞中对维生素K依赖性酶进行直接测量。
Mol Cell Biochem. 1987 May;75(1):71-6. doi: 10.1007/BF00231610.
2
Isolation and partial characterization of a vitamin K-dependent carboxylase from bovine aortae.从牛主动脉中分离并部分鉴定一种维生素K依赖性羧化酶。
Biochem J. 1987 Jul 1;245(1):251-5. doi: 10.1042/bj2450251.
3
Gamma-carboxyglutamate-containing proteins and the vitamin K-dependent carboxylase.含γ-羧基谷氨酸的蛋白质与维生素K依赖性羧化酶。
Biochem J. 1990 Mar 15;266(3):625-36. doi: 10.1042/bj2660625.