Barlas A, Sugio K, Greenbaum L M
FEBS Lett. 1985 Oct 14;190(2):268-70. doi: 10.1016/0014-5793(85)81297-7.
Plasma and inflammatory fluid kininogen levels, and blood and inflammatory fluid free kinin levels were determined in rats 24 h after the injection of carrageenin into an air pouch. Plasma T-kininogen levels increased 7-fold. In the inflammatory fluid levels reached 8 micrograms/ml. Blood levels of free kinin showed a 5-fold increase. The kinins were identified on HPLC as T-kinin (Ile-Ser-bradykinin) and bradykinin, 63 and 37%, respectively. These results indicate for the first time that free T-kinin as well as bradykinin is released during an inflammatory response in rat and confirms our previous finding that T-kininogen may be a major acute-phase protein in inflammation.
在向大鼠气囊肿内注射角叉菜胶24小时后，测定其血浆和炎性渗出液中的激肽原水平，以及血液和炎性渗出液中的游离激肽水平。血浆T-激肽原水平增加了7倍。炎性渗出液中的水平达到8微克/毫升。血液中游离激肽水平增加了5倍。通过高效液相色谱法鉴定，激肽分别为T-激肽（异亮氨酸-丝氨酸-缓激肽）和缓激肽，含量分别为63%和37%。这些结果首次表明，在大鼠的炎症反应过程中会释放游离T-激肽以及缓激肽，并证实了我们之前的发现，即T-激肽原可能是炎症中的一种主要急性期蛋白。
