利用直接机械、酶促和化学宏基因组提取的ITS和16S rDNA扩增子对糙海参肠道微生物组进行分析。
Intestinal microbiome profile of the brown rock sea cucumber () using ITS and 16S rDNA amplicons from direct mechanical, enzymatic, and chemical metagenomic extraction.
作者信息
Nieves-Morales Rene, Paez-Diaz Jessica Alejandra, Rodriguez-Carrio Sofia Marie, Melendez Martinez Gabriela, Rivera-Lopez Edwin Omar, Rodríguez-Ramos Josué, García-Arrarás José E, Rios-Velazquez Carlos
机构信息
Microbial Biotechnology and Bioprospecting Laboratory, Biology Department, University of Puerto Rico at Mayagüez, Mayagüez, USA.
Department of Food Science, The Pennsylvania State University, University Park, Pennsylvania, USA.
出版信息
Microbiol Resour Announc. 2025 Jul 10;14(7):e0029325. doi: 10.1128/mra.00293-25. Epub 2025 May 28.
Using direct mechanical, enzymatic, and chemical extraction methods, the intestinal microbiome of the marine invertebrate was obtained. ITS and 16S rDNA regions were sequenced to enrich and investigate the prokaryotic and fungal diversity profiles from different anatomical regions within the sea cucumber's intestinal biology.
采用直接机械、酶解和化学提取方法，获取了该海洋无脊椎动物的肠道微生物群。对ITS和16S rDNA区域进行测序，以富集和研究海参肠道生物学中不同解剖区域的原核生物和真菌多样性概况。
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