In vitro production of hydrogen peroxide by the amoebocytes of the scallop, Patinopecten yessoensis (Jay).

In vitro production of hydrogen peroxide (H2O2) by the amoebocytes of the scallop, Patinopecten yessoensis, was studied. The authors first confirmed the availability of the direct quantitative method using homovanillic acid, and found that both resting and stimulated amoebocytes produced H2O2. However, the latter showed higher capacity for H2O2 production. The amoebocytes stimulated by concanavalin A released a large amount of H2O2 compared with the cells challenged with three species of bacteria.