Zoletto R
Dev Biol Stand. 1985;60:179-83.
From BHK 21 cl. 13 suspensions cells we have isolated a virus serologically related to the Minute Virus of Mice (MVM); the same virus has been isolated from the same cells in U.K. in 1980. Besides isolating this virus from large scale cultures, we have also isolated it from many of the sublines stored in nitrogen we received at different times from different laboratories. At this time it is difficult to ascertain if the contamination originated from the serum or from other components of the medium; furthermore we do not know if many of the cells of our stock became contaminated in our laboratory or if they were already contaminated.
从BHK 21 cl. 13悬浮细胞中,我们分离出一种在血清学上与小鼠微小病毒(MVM)相关的病毒;1980年在英国,同样的病毒已从相同细胞中分离出来。除了从大规模培养物中分离出这种病毒外,我们还从不同时间从不同实验室收到的保存在液氮中的许多亚系中分离出了它。目前很难确定这种污染是源自血清还是培养基的其他成分;此外,我们不知道我们库存的许多细胞是在我们实验室被污染的,还是它们原本就已被污染。