Ben-Nathan D, Barzilai R, Lazar A, Shahar A
Dev Biol Stand. 1985;60:467-73.
RDB is a crude preparation extracted from a plant source. It is a very potent agent for dispersion of monolayer cell cultures as well as for the preparation of primary cell suspensions. It was found to be most effective in dispersing a wide range of animal and mosquito cells and dissociating neuronal and muscular tissue for primary cell cultures. RDB was also found to be useful for preparation of primary culture for chick embryo fibroblasts. After treatment with RDB cell viability was found to be 98% as compared to 85% in trypsin treated monolayer cultures. Routine dispersion of monolayer cultures with RDB does not have any negative effect on confluency of the newly split cultures. RDB was found to have no adverse effects on viral replication and plating efficiency of Rift Valley Fever Virus, Sindbis Virus and West Nile Virus.
RDB是一种从植物来源提取的粗制品。它是用于分散单层细胞培养物以及制备原代细胞悬液的非常有效的试剂。已发现它在分散多种动物和蚊子细胞以及解离用于原代细胞培养的神经元和肌肉组织方面最为有效。还发现RDB可用于制备鸡胚成纤维细胞的原代培养物。用RDB处理后，细胞活力为98%，而胰蛋白酶处理的单层培养物中细胞活力为85%。用RDB常规分散单层培养物对新传代培养物的汇合度没有任何负面影响。已发现RDB对裂谷热病毒、辛德毕斯病毒和西尼罗河病毒的病毒复制和接种效率没有不利影响。
