He Ping, Wang Feng, Xi Guangjun, Li Yang, Wang Fengyun, Wang Hui, Li Lei, Ma Xuemei, Han Yan, Shi Yachen
Department of Neurology, the Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center, Nanjing Medical University, Wuxi 214023, China; Department of Neurosurgery Intensive Care Unit, the Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center, Nanjing Medical University, Wuxi 214023, China.
Department of Neurology, the Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center, Nanjing Medical University, Wuxi 214023, China; Department of Interventional Neurology, the Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center, Nanjing Medical University, Wuxi 214023, China.
J Hazard Mater. 2025 Aug 15;494:138748. doi: 10.1016/j.jhazmat.2025.138748. Epub 2025 May 26.
To investigate potential associations between cerebrospinal fluid (CSF) microplastics and Alzheimer's disease (AD) pathology or cognitive function. The study included two cohorts: Cohort 1 comprised 17 amyloid-positive and 15 amyloid-negative subjects, while Cohort 2 consisted of 11 amyloid-positive subjects. All amyloid-positive participants in Cohort 1 completed one-year follow-up. CSF amyloid-β(Aβ)42, Aβ40, p-tau181, and t-tau were measured. Amyloid status defined by CSF Aβ42/40 ratio (positive: < 0.1; negative: ≥ 0.1). Four types of microplastics, namely polypropylene, polyvinyl chloride (PVC), polyethylene (PE), and polystyrene, were detected in human CSF. Bottled water consumption frequency and CSF/serum albumin ratio significantly correlated with CSF microplastic abundance. In Cohort 1, amyloid-positive subjects exhibited significantly elevated CSF levels of PE and PVC as compared to amyloid-negative subjects. In amyloid-positive subjects (Cohort 1 and 2), CSF PE levels showed significant inverse correlations with both CSF Aβ42 levels and Mini-Mental State Examination (MMSE) scores. In amyloid-positive individuals of Cohort 1, elevated PE levels positively correlated with MMSE decline rate over one year. The levels of CSF Aβ42 served as an intermediary factor, significantly affecting the relationship between CSF PE and MMSE scores in amyloid-positive subjects of cohort 1. In Cohort 1, the CSF levels of PE and PVC demonstrated an area under the curve value of greater than 0.8, indicating their strong potential to differentiate between amyloid-positive and amyloid-negative individuals. These discoveries suggested that the gradual accumulation of microplastics in the CSF were associated with cognitive decline among AD individuals.
为了研究脑脊液（CSF）中的微塑料与阿尔茨海默病（AD）病理或认知功能之间的潜在关联。该研究包括两个队列：队列1由17名淀粉样蛋白阳性和15名淀粉样蛋白阴性受试者组成，而队列2由11名淀粉样蛋白阳性受试者组成。队列1中所有淀粉样蛋白阳性参与者均完成了为期一年的随访。检测了脑脊液淀粉样蛋白β（Aβ）42、Aβ40、磷酸化tau蛋白181（p-tau181）和总tau蛋白（t-tau）。根据脑脊液Aβ42/40比值定义淀粉样蛋白状态（阳性：<0.1；阴性：≥0.1）。在人类脑脊液中检测到四种类型的微塑料，即聚丙烯、聚氯乙烯（PVC）、聚乙烯（PE）和聚苯乙烯。瓶装水饮用频率和脑脊液/血清白蛋白比值与脑脊液微塑料丰度显著相关。在队列1中，与淀粉样蛋白阴性受试者相比，淀粉样蛋白阳性受试者的脑脊液中PE和PVC水平显著升高。在淀粉样蛋白阳性受试者（队列1和队列2）中，脑脊液PE水平与脑脊液Aβ42水平和简易精神状态检查表（MMSE）评分均呈显著负相关。在队列1的淀粉样蛋白阳性个体中，PE水平升高与一年内MMSE下降率呈正相关。脑脊液Aβ42水平作为一个中介因素，显著影响队列1中淀粉样蛋白阳性受试者脑脊液PE与MMSE评分之间的关系。在队列1中，脑脊液PE和PVC水平的曲线下面积值大于0.8，表明它们在区分淀粉样蛋白阳性和阴性个体方面具有很强的潜力。这些发现表明，脑脊液中微塑料的逐渐积累与AD个体的认知衰退有关。
