绵羊红细胞与人T淋巴细胞形成玫瑰花结过程的比较研究。
A comparative study of procedures for sheep erythrocyte-human-T-lymphocyte rosette formation.
作者信息
Mahowald M L, Handwerger B S, Capertone E M, Douglas S D
出版信息
J Immunol Methods. 1977;15(3):239-45. doi: 10.1016/0022-1759(77)90061-8.
A comparative study of several published methods for sheep red blood cell-T-lymphocyte rosette formation was performed. Maximum SRBC-rosette formation occurred with AET treated SRBC in medium supplemented with 20% FCS or with untreated SRBC in 100% FCS. Prolongation of the 4 degrees C incubation period from 4 to 18 h enchanced rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette-forming lyphocytes by allowing accurate indentification of the central lymphocytes in morulas.
对几种已发表的绵羊红细胞 - T淋巴细胞玫瑰花结形成方法进行了比较研究。在补充20%胎牛血清（FCS）的培养基中，用AET处理的绵羊红细胞（SRBC）或在100% FCS中用未处理的SRBC时，出现最大程度的SRBC玫瑰花结形成。4℃孵育期从4小时延长至18小时可增强玫瑰花结形成。荧光素二乙酸酯染色显著提高了计算所得的玫瑰花结形成百分比。通过准确识别桑葚胚中的中央淋巴细胞，荧光素二乙酸酯染色显著提高了计算所得的形成玫瑰花结的淋巴细胞百分比。
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