Zhu Yanbin, Wang Jia, Zhang Xiaojuan, Cidan Yangji, Wang Hongzhuang, Pubu Panduo, Ali Munwar, Li Kun, Basang Wangdui
Institute of Animal Husbandry and Veterinary Medicine, Tibet Academy of Agriculture and Animal Husbandry Sciences, Lhasa, 850009, China.
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
Sci Rep. 2025 May 28;15(1):18610. doi: 10.1038/s41598-025-91301-8.
Traditional Chinese Medicine (TCM) is an emerging area due to increased antimicrobial resistance (AMR). The objective of this research was to explore the antioxidant, and anti-inflammatory potential of three traditional Chinese herbal formulas (TCHFs), along with variations in rumen bacteria. In this study, forty postpartum (80 ± 15) female yaks after the calves had weaned (PWFs) were divided into three experimental groups, which were offered basal feed with 5% (95% basal diet) TCHF1 (DE group), 5% TCHF2 (DF group) and 5% TCHF3 (DG group), and fourth, control group (DH group), fed only a basal diet for 30 days. Following blood and rumen fluid samples on the 15th and 30th day, ELISA testing was performed to check antioxidant enzyme levels and inflammatory mediators. The results indicated that TCHF2 significantly upregulated the interleukin-10 (IL-10) (p < 0.05). Additionally, 16 S rRNA sequencing results showed that TCHF2 significantly enhanced Firmicutes to Bacteroidetes ratio (F/B) at the phylum level. On day 15th, phylum Actinobacteria, SR1, Cyanobacteria, and Armatimonadetes were found to be significantly (p < 0.05) different, while, at the genus level, Butyrivibrio, CF231, YRC22, Moraxella, Clostridium, etc. were significantly different (p < 0.05). On day 30, phylum SR1, Armatimonadetes, Chlorobi, and genus Coprococcus, Oscillospira, Selenomonas, L7A_E11, Clostridium, etc. were found to be significantly different (p < 0.05). This study concluded that TCHF2 is the most effective one among all.
由于抗菌药物耐药性（AMR）的增加，传统中医（TCM）成为一个新兴领域。本研究的目的是探讨三种传统中药配方（TCHFs）的抗氧化和抗炎潜力，以及瘤胃细菌的变化。在本研究中，四十头产后（80±15）且小牛已断奶的雌性牦牛（PWFs）被分为三个实验组，分别给它们提供添加5%（95%基础日粮）TCHF1的基础饲料（DE组）、5% TCHF2（DF组）和5% TCHF3（DG组），第四组为对照组（DH组），仅饲喂基础日粮30天。在第15天和第30天采集血液和瘤胃液样本后，进行酶联免疫吸附测定（ELISA）检测以检查抗氧化酶水平和炎症介质。结果表明，TCHF2显著上调白细胞介素10（IL-10）（p<0.05）。此外，16S rRNA测序结果显示，TCHF2在门水平上显著提高了厚壁菌门与拟杆菌门的比例（F/B）。在第15天，放线菌门、SR1、蓝细菌门和装甲菌门被发现有显著差异（p<0.05），而在属水平上，丁酸弧菌属、CF231、YRC22、莫拉克斯氏菌属、梭菌属等有显著差异（p<0.05）。在第30天，SR1门、装甲菌门、绿弯菌门以及粪球菌属、颤螺菌属、硒单胞菌属、L7A_E11、梭菌属等被发现有显著差异（p<0.05）。本研究得出结论，TCHF2是所有配方中最有效的一种。
