College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
College of Chemistry and Life Sciences, Gansu Minzu Normal University, Gannan, China.
Front Cell Infect Microbiol. 2024 Aug 23;14:1443712. doi: 10.3389/fcimb.2024.1443712. eCollection 2024.
To investigate the effects of Shen Qi Bu Qi Powder (SQBQP) on the average daily gain, blood indexes, gastrointestinal microflora, and serum metabolites of calves.
A total of 105 calves were randomly assigned to three groups (n = 35 per group): the control group (C, fed with a basal diet for 21 days) and two treatment groups (SQBQP-L and SQBQP-H, fed with the basal diet supplemented with 15 and 30 g/kg of SQBQP), respectively for 21 days. The active components of SQBQP were identified using LC-MS/MS. Serum digestive enzymes and antioxidant indices were determined by ELISA kits and biochemical kits, respectively. Serum differential metabolites were analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS), while flora in rumen fluid and fecal were analyzed by 16S rDNA sequencing. Further correlation analysis of gastrointestinal flora and serum metabolites of SQBQP-H and C groups were performed with Spearman's correlation.
The principal active components of SQBQP mainly includes polysaccharides, flavonoids, and organic acids. Compared to the control group (C), calves in the SQBQP-H (high dose) and SQBQP-L (low dose) groups showed a significant increase in serum amylase (AMS) levels (<0.001), while lipase content significantly decreased (<0.05). Additionally, the average daily gain, T-AOC, and cellulase content of calves in the SQBQP-H group significantly increased (<0.05). and in the rumen flora of the SQBQP-H group was significantly lower than that of the C group (<0.05). The relative abundance of , and in the SQBQP-H group was significantly increased (<0.05), while the relative abundance of and was significantly decreased (<0.05). Serum metabolomics analysis revealed 20 differential metabolites, mainly enriched in amino acid biosynthesis, β-alanine metabolism, tyrosine, and tryptophan biosynthesis metabolic pathways (<0.05). Correlation analysis results showed that in rumen flora and in intestinal flora were significantly positively correlated with average daily gain, serum biochemical indexes, and differential metabolite (-)-Epigallocatechin (R>0.58, <0.05).
SQBQP can promote calves weight gain and enhance health by modulating gastrointestinal flora and metabolic processes in the body.
研究参芪补气散对犊牛平均日增重、血液指标、胃肠道微生物区系和血清代谢物的影响。
将 105 头犊牛随机分为三组（每组 35 头）：对照组（C，基础日粮喂养 21 天）和两个治疗组（SQBQP-L 和 SQBQP-H，分别在基础日粮中添加 15 和 30 g/kg 的 SQBQP），分别喂养 21 天。采用 LC-MS/MS 鉴定 SQBQP 的活性成分。采用 ELISA 试剂盒和生化试剂盒分别测定血清消化酶和抗氧化指标。采用液相色谱-质谱/质谱（LC-MS/MS）分析血清差异代谢物，采用 16S rDNA 测序分析瘤胃液和粪便中的菌群。采用 Spearman 相关性分析对 SQBQP-H 组和 C 组的胃肠道菌群和血清代谢物进行相关性分析。
SQBQP 的主要活性成分主要包括多糖、黄酮类化合物和有机酸。与对照组（C）相比，SQBQP-H（高剂量）和 SQBQP-L（低剂量）组犊牛血清淀粉酶（AMS）水平显著升高（<0.001），而脂肪酶含量显著降低（<0.05）。此外，SQBQP-H 组犊牛的平均日增重、T-AOC 和纤维素酶含量均显著升高（<0.05）。SQBQP-H 组瘤胃菌群中的显著低于 C 组（<0.05）。SQBQP-H 组中的相对丰度显著增加（<0.05），而和的相对丰度显著降低（<0.05）。血清代谢组学分析显示 20 个差异代谢物，主要富集在氨基酸生物合成、β-丙氨酸代谢、酪氨酸和色氨酸生物合成代谢途径（<0.05）。相关性分析结果表明，瘤胃菌群中的和肠道菌群中的与平均日增重、血清生化指标和差异代谢物（-）-表没食子儿茶素（R>0.58，<0.05）呈显著正相关。
SQBQP 通过调节犊牛胃肠道菌群和体内代谢过程，促进犊牛增重，增强健康。
