Hernández J, Mesa F, Riveros A, Fayad R, Nisperuza J
Departamento de Biología, Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, Colombia.
NanoTech Group, Facultad de Ingeniería y Ciencias Básicas, Fundación Universitaria Los, Libertadores, Cra 16 No. 63a-68, Bogotá 111221, Colombia.
Integr Org Biol. 2025 May 9;7(1):obaf018. doi: 10.1093/iob/obaf018. eCollection 2025.
This study developed and optimized a methodology based on controlled centrifugation for the segmented extraction of extracellular fluid in bees. Three critical variables were analyzed: relative centrifugal force, centrifugation time, and the number of individuals processed, ensuring the reproducibility and efficiency of the procedure. The results demonstrated significant differences in the volume of fluid recovered from different body segments, with the abdomen yielding the highest volumes, followed by the thorax and the head. UV-Vis spectroscopic characterization revealed distinct optical features for the samples, identifying specific absorbance peaks unique to each segment. Furthermore, biochemical analysis using Benedict's reagent confirmed the presence of reducing sugars, with head samples displaying the most intense coloration. These findings underscore the importance of segment-specific analysis to gain deeper insights into the physiology and metabolism of bees. The proposed methodology offers a novel and robust tool for physiological, metabolic, and ecotoxicological studies, facilitating the assessment of environmental and contaminant impacts on pollinator health.
本研究开发并优化了一种基于控制离心的方法，用于分段提取蜜蜂的细胞外液。分析了三个关键变量：相对离心力、离心时间和处理的个体数量，以确保该程序的可重复性和效率。结果表明，从不同身体部位回收的液体体积存在显著差异，腹部回收的液体体积最大，其次是胸部和头部。紫外可见光谱表征揭示了样品独特的光学特征，确定了每个部位特有的特定吸收峰。此外，使用本尼迪克特试剂进行的生化分析证实了还原糖的存在，头部样品的颜色最深。这些发现强调了特定部位分析对于深入了解蜜蜂生理和代谢的重要性。所提出的方法为生理、代谢和生态毒理学研究提供了一种新颖且强大的工具，有助于评估环境和污染物对传粉者健康的影响。
