Zhang Bo-Wen, Yang Bai-Song, Wan Xiao-Neng, Ma Xin, Lyu Kai-Di, Wang Han, Yang Shu-Ying, Zhang Hui-Hui, Hao Shu-Nv, Ma Jian, Sun Guo-Zhong
College of Agronomy, Jilin Agricultural University, Changchun, China.
National Engineering Research Center for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
Front Plant Sci. 2025 May 14;16:1594385. doi: 10.3389/fpls.2025.1594385. eCollection 2025.
Pre-harvest sprouting (PHS) is a significant challenge affecting global production of wheat ( L.). Resistance to PHS is governed by both genetic and environmental factors, making reliable molecular markers essential for enhancing PHS resistance through molecular marker-assisted selection (MAS). Genes and have been cloned in wheat and are known to regulate PHS resistance in white-grained varieties. In this study, we assessed the allelic variations in these genes and their combined effects on PHS resistance using two recombinant inbred line (RIL) populations, Wanxianbaimaizi/Zhongyou 9507 (WZ) and Wanxianbaimaizi/Jing 411 (WJ), under two distinct field environmental conditions. PHS resistance was assessed by measuring seed germination in physiologically mature stage, correlation and ANOVA were used to analyze PHS data. The germination percentage (GP) and germination index (GI) were significantly correlated across both RIL populations. Specific allelic variations at positions -222, +646, and +666 in the gene strongly correlated with PHS resistance. The CGA haplotype at these loci was linked to the highest resistance, while the TAA haplotype was associated with the lowest resistance levels. Additionally, haplotype variation at the +660 locus of demonstrated a weak but environmentally modulated correlation with PHS resistance. This study provides a theoretical foundation for utilizing and in molecular breeding strategies to enhance wheat resilience to PHS.
收获前发芽（PHS）是影响全球小麦（L.）产量的一个重大挑战。对PHS的抗性受遗传和环境因素共同影响，这使得可靠的分子标记对于通过分子标记辅助选择（MAS）提高PHS抗性至关重要。小麦中的基因 和 已被克隆，并且已知它们在白粒品种中调节PHS抗性。在本研究中，我们在两种不同的田间环境条件下，使用两个重组自交系（RIL）群体，即万县白麦子/中优9507（WZ）和万县白麦子/京411（WJ），评估了这些基因的等位变异及其对PHS抗性的综合影响。通过测量生理成熟阶段的种子发芽率来评估PHS抗性，使用相关性分析和方差分析来分析PHS数据。两个RIL群体的发芽率（GP）和发芽指数（GI）均显著相关。基因 中 -222、+646和 +666位点的特定等位变异与PHS抗性密切相关。这些位点的CGA单倍型与最高抗性相关，而TAA单倍型与最低抗性水平相关。此外，基因 的 +660位点的单倍型变异与PHS抗性呈现出较弱但受环境调节的相关性。本研究为在分子育种策略中利用基因 和 提高小麦对PHS的抗性提供了理论基础。
