Reis Adriano, Carvalho Caroline Pais, de Lima Iasmim Lopes, Mansoldo Felipe R P, Vermelho Alane Beatriz, Simas Rosineide Costa, Eberlin Livia S, Eberlin Marcos N
School of Engineering, Mackenzie Presbyterian University, São Paulo, São Paulo, Brazil.
MackGraphe-Mackenzie Institute for Research in Graphene and Nanotechnologies, Mackenzie Presbyterian Institute, São Paulo, São Paulo, Brazil.
J Mass Spectrom. 2025 Jun;60(6):e5133. doi: 10.1002/jms.5133.
We have investigated the ability of a MasSpec Pen (MSPen) "three-in-one" (extraction, transfer, and ionization) device coupled to a mass spectrometer to provide instantaneous chemical profiles that could promptly characterize wood samples from the mahogany (Meliaceae) family. For that, we selected a set of five representative wood species, that is, Brazilian mahogany (Swietenia macrophylla, also known as Honduran mahogany), two African mahoganies (Khaya ivorensis and Khaya senegalensis), and two "nongenuine" ("fake") mahogany woods: cedar (Cedrela odorata) and andiroba (Carapa guianensis). By simply touching the superficially polished wood surface and after 3 s of automatic extraction, profiles of highly characteristic markers that effectively discriminated all five mahoganies were detected. The superficial surface of a wood Brazilian mahogany sample as compared with internal wood accessed via deep sanding showed minor profile changes mainly by shifts in the relative abundances of the wood markers, indicating that aging only marginally changes MSPen wood signatures. The direct "touch&play" analysis offered by MSPen was therefore found to provide nondestructive, fast, sample-preparation-free, and reliable typification of woods. This "spatially free" device also allows broad screening because multiple points on the whole surface of any small or large-size intricate wood sample can be rapidly analyzed, demonstrating its high potential for forensic investigations, particularly for endangered species such as the Brazilian mahogany.
我们研究了一种与质谱仪相连的MasSpec Pen（MSPen）“三合一”（提取、转移和电离）设备提供即时化学图谱的能力，该图谱可迅速表征楝科桃花心木属的木材样本。为此，我们挑选了一组五个具有代表性的木材物种，即巴西桃花心木（大叶桃花心木，又称洪都拉斯桃花心木）、两种非洲桃花心木（非洲楝和塞内加尔非洲楝）以及两种“非正宗”（“假”）桃花心木：雪松（洋椿）和红苦楝（圭亚那卡拉帕树）。只需简单触摸表面打磨过的木材表面，经过3秒自动提取后，就能检测到有效区分所有这五种桃花心木的高度特征性标志物的图谱。与通过深度打磨获取的内部木材相比，巴西桃花心木样本的木材表面图谱变化较小，主要是木材标志物相对丰度的变化，这表明老化对MSPen木材特征的影响微乎其微。因此，发现MSPen提供的直接“触摸即分析”能对木材进行无损、快速、无需样品制备且可靠的分类。这种“空间自由”的设备还允许进行广泛筛选，因为任何大小复杂的木材样本整个表面的多个点都能快速分析，显示出其在法医调查中的巨大潜力，特别是对于像巴西桃花心木这样的濒危物种。
