Falke N E, Fischer E G
Immunobiology. 1985 Jul;169(5):532-9. doi: 10.1016/S0171-2985(85)80007-3.
The effects of beta-endorphin(beta-End), an endogenous opioid, were tested in vitro on shape changes in polymorphonuclear leukocytes (PMNs). Cell shape changes indicate alterations of the functional status of the cells. Within 2 min, beta-End but not the opioid alkaloid levorphanol or the antagonist, diprenorphine, induced a cell spreading. Subsequently, beta-End and levorphanol (10(-8) M), but not the dextrorotatory isomer, stimulated an elongation of the cells. Both effects of beta-End could be antagonized by diprenorphine in an equimolar concentration. Thus, the effects were stereo-specific and antagonizable. In this test system, the morphological changes evoked by beta-End were equal to the effects of FMLP, a chemotactic substance, used as a reference. Our findings indicate that endogenous opioids might play a role in modulating the initial phase of the PMNs' offensive behaviour, presumably cell adherence and motility.
内源性阿片样物质β-内啡肽（β-End）对多形核白细胞（PMN）形态变化的影响在体外进行了测试。细胞形态变化表明细胞功能状态的改变。在2分钟内，β-内啡肽而非阿片生物碱左啡诺或拮抗剂二丙诺啡诱导细胞铺展。随后，β-内啡肽和左啡诺（10^(-8) M），但右旋异构体无此作用，刺激细胞伸长。β-内啡肽的这两种作用均可被等摩尔浓度的二丙诺啡拮抗。因此，这些作用具有立体特异性且可被拮抗。在该测试系统中，β-内啡肽引起的形态变化与用作参照的趋化物质FMLP的作用相当。我们的研究结果表明，内源性阿片样物质可能在调节PMN攻击行为的初始阶段发挥作用，推测是细胞黏附和运动。
