一种适用于mRNA疫苗质量控制的双链RNA检测改进方法。 - Suppr

An improved method for the detection of double-stranded RNA suitable for quality control of mRNA vaccines.

作者信息

Liu Jingjing, Zheng Tao, Xu Lingjie, Chen Zhicai, Zhang Kunkun, Wang Xiangxi, Xu Xiaoyu, Li Yuhua, Sun Yao, Zhu Ling

机构信息

Department of Arbovirus Vaccine, National Institutes for Food and Drug Control, Beijing 102629, China.

CAS Key Laboratory of Infection and Immunity, National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

出版信息

Protein Cell. 2024 Nov 1;15(11):791-795. doi: 10.1093/procel/pwae043.

DOI:10.1093/procel/pwae043

PMID:40440055

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11528662/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/264d/11528662/c42c43364c02/pwae043_fig1.jpg

相似文献

An improved method for the detection of double-stranded RNA suitable for quality control of mRNA vaccines.

Protein Cell. 2024 Nov 1;15(11):791-795. doi: 10.1093/procel/pwae043.

A microfluidic electrophoretic dual dynamic staining method for the identification and relative quantitation of dsRNA contaminants in mRNA vaccines.

Analyst. 2023 Aug 7;148(16):3758-3767. doi: 10.1039/d3an00281k.

Designing immunostimulatory double stranded messenger RNA with maintained translational activity through hybridization with poly A sequences for effective vaccination.

Biomaterials. 2018 Jan;150:162-170. doi: 10.1016/j.biomaterials.2017.09.033. Epub 2017 Sep 27.

Development of Biolayer Interferometry (BLI)-Based Double-Stranded RNA Detection Method with Application in mRNA-Based Therapeutics and Vaccines.

Pharmaceutics. 2024 Sep 19;16(9):1227. doi: 10.3390/pharmaceutics16091227.

Engineered mRNAs With Stable Structures Minimize Double-stranded RNA Formation and Increase Protein Expression.

J Mol Biol. 2024 Nov 15;436(22):168822. doi: 10.1016/j.jmb.2024.168822. Epub 2024 Oct 19.

Double-stranded RNA reduction by chaotropic agents during transcription of messenger RNA.

Mol Ther Nucleic Acids. 2022 Aug 4;29:618-624. doi: 10.1016/j.omtn.2022.08.001. eCollection 2022 Sep 13.

Research progress on immune mechanism and control strategy of dsRNA impurities in mRNA vaccine.

Expert Rev Vaccines. 2025 Dec;24(1):457-469. doi: 10.1080/14760584.2025.2510335. Epub 2025 Jun 2.

[mRNA sequences complementary to the double-stranded regions of pre-mRNA. Hybridization experiments using RNA mercuriation and chromatography on sulfhydryl-Sepharose].

Mol Biol (Mosk). 1979 May-Jun;13(3):601-12.

Monoclonal antibody assay for detection of double-stranded RNA and application for detection of group A and non-group A rotaviruses.

J Clin Microbiol. 1989 Jan;27(1):6-12. doi: 10.1128/jcm.27.1.6-12.1989.

Vaccines' New Era-RNA Vaccine.

Viruses. 2023 Aug 18;15(8):1760. doi: 10.3390/v15081760.

引用本文的文献

c-di-GMP does not bind H-NS, nor inhibits H-NS binding DNA.

Nat Commun. 2025 Jun 12;16(1):5287. doi: 10.1038/s41467-025-60689-2.

本文引用的文献

An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts.

Nat Biotechnol. 2023 Apr;41(4):560-568. doi: 10.1038/s41587-022-01525-6. Epub 2022 Nov 10.

Immunogenicity of -Transcribed RNA.

Acc Chem Res. 2021 Nov 2;54(21):4012-4023. doi: 10.1021/acs.accounts.1c00521. Epub 2021 Oct 22.

Detailed Dissection and Critical Evaluation of the Pfizer/BioNTech and Moderna mRNA Vaccines.

Vaccines (Basel). 2021 Jul 3;9(7):734. doi: 10.3390/vaccines9070734.

Coronavirus Disease 2019 Vaccine Development: An Overview.

Viral Immunol. 2021 Apr;34(3):134-144. doi: 10.1089/vim.2020.0119. Epub 2020 Sep 23.

Characteristics of and Public Health Responses to the Coronavirus Disease 2019 Outbreak in China.

J Clin Med. 2020 Feb 20;9(2):575. doi: 10.3390/jcm9020575.

Identification of the long, edited dsRNAome of LPS-stimulated immune cells.

Genome Res. 2016 Jun;26(6):852-62. doi: 10.1101/gr.203992.116. Epub 2016 Apr 21.

NPDock: a web server for protein-nucleic acid docking.

Nucleic Acids Res. 2015 Jul 1;43(W1):W425-30. doi: 10.1093/nar/gkv493. Epub 2015 May 14.

The double-stranded transcriptome of Escherichia coli.

Proc Natl Acad Sci U S A. 2014 Feb 25;111(8):3134-9. doi: 10.1073/pnas.1315974111. Epub 2014 Jan 22.

Detection of Leishmania RNA virus in Leishmania parasites.

PLoS Negl Trop Dis. 2013;7(1):e2006. doi: 10.1371/journal.pntd.0002006. Epub 2013 Jan 10.

The role of UNC93B1 protein in surface localization of TLR3 receptor and in cell priming to nucleic acid agonists.

J Biol Chem. 2013 Jan 4;288(1):442-54. doi: 10.1074/jbc.M112.413922. Epub 2012 Nov 19.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验

An improved method for the detection of double-stranded RNA suitable for quality control of mRNA vaccines.

作者信息

Liu Jingjing, Zheng Tao, Xu Lingjie, Chen Zhicai, Zhang Kunkun, Wang Xiangxi, Xu Xiaoyu, Li Yuhua, Sun Yao, Zhu Ling

机构信息

Department of Arbovirus Vaccine, National Institutes for Food and Drug Control, Beijing 102629, China.

CAS Key Laboratory of Infection and Immunity, National Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

出版信息

Protein Cell. 2024 Nov 1;15(11):791-795. doi: 10.1093/procel/pwae043.

DOI:10.1093/procel/pwae043

PMID:40440055

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11528662/

Abstract

摘要

相似文献

An improved method for the detection of double-stranded RNA suitable for quality control of mRNA vaccines.

Protein Cell. 2024 Nov 1;15(11):791-795. doi: 10.1093/procel/pwae043.

A microfluidic electrophoretic dual dynamic staining method for the identification and relative quantitation of dsRNA contaminants in mRNA vaccines.

Analyst. 2023 Aug 7;148(16):3758-3767. doi: 10.1039/d3an00281k.

Designing immunostimulatory double stranded messenger RNA with maintained translational activity through hybridization with poly A sequences for effective vaccination.

Biomaterials. 2018 Jan;150:162-170. doi: 10.1016/j.biomaterials.2017.09.033. Epub 2017 Sep 27.

Development of Biolayer Interferometry (BLI)-Based Double-Stranded RNA Detection Method with Application in mRNA-Based Therapeutics and Vaccines.

Pharmaceutics. 2024 Sep 19;16(9):1227. doi: 10.3390/pharmaceutics16091227.

Engineered mRNAs With Stable Structures Minimize Double-stranded RNA Formation and Increase Protein Expression.

J Mol Biol. 2024 Nov 15;436(22):168822. doi: 10.1016/j.jmb.2024.168822. Epub 2024 Oct 19.

Double-stranded RNA reduction by chaotropic agents during transcription of messenger RNA.

Mol Ther Nucleic Acids. 2022 Aug 4;29:618-624. doi: 10.1016/j.omtn.2022.08.001. eCollection 2022 Sep 13.

Research progress on immune mechanism and control strategy of dsRNA impurities in mRNA vaccine.

Expert Rev Vaccines. 2025 Dec;24(1):457-469. doi: 10.1080/14760584.2025.2510335. Epub 2025 Jun 2.

[mRNA sequences complementary to the double-stranded regions of pre-mRNA. Hybridization experiments using RNA mercuriation and chromatography on sulfhydryl-Sepharose].

Mol Biol (Mosk). 1979 May-Jun;13(3):601-12.

Monoclonal antibody assay for detection of double-stranded RNA and application for detection of group A and non-group A rotaviruses.

J Clin Microbiol. 1989 Jan;27(1):6-12. doi: 10.1128/jcm.27.1.6-12.1989.

Vaccines' New Era-RNA Vaccine.

Viruses. 2023 Aug 18;15(8):1760. doi: 10.3390/v15081760.

引用本文的文献

c-di-GMP does not bind H-NS, nor inhibits H-NS binding DNA.

Nat Commun. 2025 Jun 12;16(1):5287. doi: 10.1038/s41467-025-60689-2.

本文引用的文献

An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts.

Nat Biotechnol. 2023 Apr;41(4):560-568. doi: 10.1038/s41587-022-01525-6. Epub 2022 Nov 10.

Immunogenicity of -Transcribed RNA.

Acc Chem Res. 2021 Nov 2;54(21):4012-4023. doi: 10.1021/acs.accounts.1c00521. Epub 2021 Oct 22.

Detailed Dissection and Critical Evaluation of the Pfizer/BioNTech and Moderna mRNA Vaccines.

Vaccines (Basel). 2021 Jul 3;9(7):734. doi: 10.3390/vaccines9070734.

Coronavirus Disease 2019 Vaccine Development: An Overview.

Viral Immunol. 2021 Apr;34(3):134-144. doi: 10.1089/vim.2020.0119. Epub 2020 Sep 23.

Characteristics of and Public Health Responses to the Coronavirus Disease 2019 Outbreak in China.

J Clin Med. 2020 Feb 20;9(2):575. doi: 10.3390/jcm9020575.

Identification of the long, edited dsRNAome of LPS-stimulated immune cells.

Genome Res. 2016 Jun;26(6):852-62. doi: 10.1101/gr.203992.116. Epub 2016 Apr 21.

NPDock: a web server for protein-nucleic acid docking.

Nucleic Acids Res. 2015 Jul 1;43(W1):W425-30. doi: 10.1093/nar/gkv493. Epub 2015 May 14.

The double-stranded transcriptome of Escherichia coli.

Proc Natl Acad Sci U S A. 2014 Feb 25;111(8):3134-9. doi: 10.1073/pnas.1315974111. Epub 2014 Jan 22.

Detection of Leishmania RNA virus in Leishmania parasites.

PLoS Negl Trop Dis. 2013;7(1):e2006. doi: 10.1371/journal.pntd.0002006. Epub 2013 Jan 10.

The role of UNC93B1 protein in surface localization of TLR3 receptor and in cell priming to nucleic acid agonists.

J Biol Chem. 2013 Jan 4;288(1):442-54. doi: 10.1074/jbc.M112.413922. Epub 2012 Nov 19.

An improved method for the detection of double-stranded RNA suitable for quality control of mRNA vaccines.

作者信息

机构信息

出版信息

相似文献

引用本文的文献

本文引用的文献

文献AI研究员

用中文搜PubMed

文档翻译

Suppr 超能文献

An improved method for the detection of double-stranded RNA suitable for quality control of mRNA vaccines.

作者信息

机构信息

出版信息

相似文献

引用本文的文献

本文引用的文献