在内皮细胞存在的情况下，人类多形核白细胞对葡萄球菌的吞噬作用会增强。
Phagocytosis of staphylococci by human polymorphonuclear leukocytes is enhanced in the presence of endothelial cells.
作者信息
Vandenbroucke-Grauls C M, Thijssen H M, Verhoef J
出版信息
Infect Immun. 1985 Oct;50(1):250-4. doi: 10.1128/iai.50.1.250-254.1985.
The role of various surfaces in the phagocytosis of Staphylococcus aureus by human polymorphonuclear leukocytes (PMN) was studied. Uptake of both opsonized and unopsonized staphylococci on the surface of a monolayer of human venous endothelial cells was compared with uptake on an inert plastic surface, with an assay that uses radiolabeled bacteria. Uptake of unopsonized S. aureus was threefold higher on the endothelial cell surface than on the plastic surface and was followed by efficient killing of the phagocytosed staphylococci. Uptake of unopsonized S. aureus on endothelial cells was not inhibited by treatment of the PMN with pronase or 2-deoxy-D-glucose and was only partially inhibited by cytochalasin B treatment of the PMN. The supporting effect of endothelial cells on the phagocytosis of unopsonized S. aureus was not due to opsonization of the bacteria by immunoglobulin or complement from the endothelial cell surface, nor to coating with fibronectin.
研究了人体多形核白细胞（PMN）吞噬金黄色葡萄球菌过程中各种表面的作用。采用放射性标记细菌的检测方法，将调理素化和未调理素化的葡萄球菌在人静脉内皮细胞单层表面的摄取情况与在惰性塑料表面的摄取情况进行了比较。未调理素化的金黄色葡萄球菌在内皮细胞表面的摄取量比在塑料表面高3倍，随后被吞噬的葡萄球菌被有效杀灭。用链霉蛋白酶或2-脱氧-D-葡萄糖处理PMN后，未调理素化的金黄色葡萄球菌在内皮细胞上的摄取不受抑制，而用细胞松弛素B处理PMN后，摄取仅部分受抑制。内皮细胞对未调理素化的金黄色葡萄球菌吞噬的支持作用，既不是由于内皮细胞表面的免疫球蛋白或补体对细菌进行调理，也不是由于纤连蛋白的包被。
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