Berthier-Vergnes O, Portoukalian J, Doré J F
Int J Cancer. 1985 Oct 15;36(4):461-6. doi: 10.1002/ijc.2910360408.
Human malignant melanoma cell lines characterized by either a high or a low ability to grow subcutaneously in athymic nude mice have been examined for their cell-surface glycoproteins. Striking differences were demonstrated between these 2 groups. Cells from lines of low tumorigenicity (LT group) displayed twice as much Vibrio cholerae neuraminidase and galactose oxidase accessible glycoproteins as cells from lines of high tumorigenicity (HT group) and each group of cell lines could be characterized by specific glycoprotein profiles. LT and HT group cells displayed similar amounts of periodate accessible glycoproteins, but sialoglycoprotein profiles were characteristic for each group of cell lines. Furthermore, whereas 87% of the sialic acid released by V. cholerae neuraminidase came from cell surface glycoproteins in HT group cells, only 53-55% of the released sialic acid came from surface glycoproteins in LT group cells. These results suggest that human melanoma cell lines exhibiting different tumorigenicity in nude mice can also be characterized by differences in composition and organization within the plasma membranes of their cell-surface sialoglycoproteins.
已对在无胸腺裸鼠皮下生长能力有高有低的人恶性黑色素瘤细胞系进行了细胞表面糖蛋白检测。这两组细胞系之间表现出显著差异。低致瘤性细胞系（LT组）的细胞所显示的可被霍乱弧菌神经氨酸酶和半乳糖氧化酶作用的糖蛋白量是高致瘤性细胞系（HT组）细胞的两倍，并且每组细胞系都有特定的糖蛋白谱特征。LT组和HT组细胞的高碘酸盐可作用糖蛋白量相似，但唾液酸糖蛋白谱对每组细胞系来说都具有特征性。此外，虽然霍乱弧菌神经氨酸酶释放的唾液酸中有87%来自HT组细胞的细胞表面糖蛋白，但LT组细胞中释放的唾液酸只有53 - 55%来自表面糖蛋白。这些结果表明，在裸鼠中表现出不同致瘤性的人黑色素瘤细胞系，其细胞表面唾液酸糖蛋白的质膜组成和组织差异也可作为其特征。
