Szerdahelyi P, Kása P
Histochemistry. 1985;83(2):181-7. doi: 10.1007/BF00495151.
Histochemical and atomic absorption spectrophotometric methods were used to study the effects of reserpine and d-amphetamine on the neuronal trace metal distribution in various regions of the central nervous system (hippocampus, parietal cortex, cerebellum). Six hours after single d-amphetamine treatment (15 mg/kg i.p.), the neuronal zinc level was significantly decreased in the hippocampus and in the parietal cortex. The intensity of sulphide silver staining was similarly greatly decreased in all layers of the hippocampus and the parietal cortex. Such a change was not observed when d-amphetamine was administered in a lower dose (5 or 10 mg/kg i.p.). Twenty hours after single reserpine treatment (10 mg/kg i.p.), there were no changes in the tissue levels and distribution of zinc, copper, iron and manganese. In animals treated with reserpine on five consecutive days, in a dose of 10 mg/kg/day i.p., the trace metal distribution twenty hours following the final treatment was essentially the same as in the control. The results strongly suggest that zinc does not play a direct role in vivo in the storage and mobilization processes of the catecholamines. A high dose of d-amphetamine, however, has a non-specific, toxic effect that is not interrelated with the catecholaminergic neuronal function; this effect is manifested in a diminished intensity of sulphide silver staining and in a reduction of the tissue zinc level.
采用组织化学和原子吸收分光光度法研究利血平和d-苯丙胺对中枢神经系统不同区域（海马、顶叶皮质、小脑）神经元微量金属分布的影响。单次腹腔注射d-苯丙胺（15mg/kg）6小时后，海马和顶叶皮质的神经元锌水平显著降低。海马和顶叶皮质各层的硫化银染色强度也同样大幅降低。当以较低剂量（5或10mg/kg腹腔注射）给予d-苯丙胺时，未观察到这种变化。单次腹腔注射利血平（10mg/kg）20小时后，锌、铜、铁和锰的组织水平及分布没有变化。连续5天腹腔注射利血平（10mg/kg/天）的动物，在最后一次给药20小时后的微量金属分布与对照组基本相同。结果强烈表明，锌在体内对儿茶酚胺的储存和动员过程不发挥直接作用。然而，高剂量的d-苯丙胺具有非特异性的毒性作用，与儿茶酚胺能神经元功能无关；这种作用表现为硫化银染色强度减弱和组织锌水平降低。
