Chen Guoqi, An Kai, Xue Jiahao, Deng Wei, Huang Zeyue, Xu Yang
Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou, China.
Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, China.
Pest Manag Sci. 2025 Sep;81(9):5589-5601. doi: 10.1002/ps.8927. Epub 2025 May 30.
Various mechanisms or factors may cause the wide infestation of herbicide-resistant weeds with different contributions, such as target-site resistance (TSR), non-target-site resistance (NTSR), hormesis, and dissemination of herbicide-resistant weeds. Here we tested this hypothesis in eastern China in Leptochloa chinensis, for which cyhalofop resistance is a major challenge to rice planting.
Our results revealed that 30% of the total 242 populations held high (10 < RI < 100) or very high resistance (RI > 100) to cyhalofop. Resistance increased from north to south, and from transplanted rice fields to direct-seeded rice fields. Six mutant types conferring TSR to cyhalofop were found in 89% sampled populations, with 18% carrying two types, 2% carrying three types, and another 2% carrying four types of mutations. W2027C was the most frequent TSR mutation, present in 47.3% of sampled populations, followed by I2041N (21.8%), W1999C (20%), L1818F (20%), W1999G (5.5%) and C2088R (1.8%). Non-target-site resistance linked with P450 monooxygenases (P450) and glutathione S-transferases (GST) were observed in 7% and 10% cyhalofop-resistant populations sampled. No significant correlation between genetic and geographic distances were observed among cyhalofop-resistant populations. Hormetic effects of cyhalofop to L. chinensis were evident in 13% of overall populations.
This study compared the contribution of different mechanisms towards herbicide resistance, revealed herbicide hormesis and revealed the distribution of resistance. Insights from this study could be important for weed management practices in other areas of the world. © 2025 Society of Chemical Industry.
多种机制或因素可能导致抗除草剂杂草广泛传播，其作用各不相同，如靶标位点抗性（TSR）、非靶标位点抗性（NTSR）、兴奋效应以及抗除草剂杂草的扩散。在此，我们在中国东部对稗草进行了该假设的验证，对水稻种植而言，抗氰氟草酯是一个重大挑战。
我们的结果显示，在总共242个种群中，30%对氰氟草酯具有高抗性（10＜抗性指数＜100）或极高抗性（抗性指数＞100）。抗性从北向南、从移栽稻田到直播稻田呈上升趋势。在89%的抽样种群中发现了六种赋予对氰氟草酯靶标位点抗性的突变类型，其中18%携带两种突变类型，2%携带三种突变类型，另有2%携带四种突变类型。W2027C是最常见的靶标位点抗性突变，存在于47.3%的抽样种群中，其次是I2041N（21.8%）、W1999C（20%）、L1818F（20%）、W1999G（5.5%）和C2088R（1.8%）。在7%的抗氰氟草酯抽样种群中观察到与细胞色素P450单加氧酶（P450）和谷胱甘肽S - 转移酶（GST）相关的非靶标位点抗性。在抗氰氟草酯种群中，未观察到遗传距离与地理距离之间存在显著相关性。氰氟草酯对稗草的兴奋效应在13%的总体种群中很明显。
本研究比较了不同机制对除草剂抗性的贡献，揭示了除草剂兴奋效应并揭示了抗性分布情况。本研究的见解可能对世界其他地区的杂草管理实践具有重要意义。©2025化学工业协会。
