Adam Ladislaus Tapiwa, Pepper Michael S, Ambele Melvin A
Institute for Cellular and Molecular Medicine, Department of Medical Immunology, and SAMRC Extramural Unit for Stem Cell Research and Therapy, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Methods Mol Biol. 2025;2938:115-118. doi: 10.1007/978-1-0716-4607-6_13.
Adipose-derived stromal/stem cells (ASCs) have generated great interest both with regard to research and their potential applications in translational clinical sciences. ASCs are often required for use at a later time relative to the time at which they were harvested. This requires appropriate conditions and procedures for storage and retrieval of these cells as needed. This protocol describes cryopreservation and thawing of ASCs without compromising the viability of the stored cells.
脂肪来源的基质/干细胞（ASC）在研究及其在转化临床科学中的潜在应用方面都引起了极大的兴趣。相对于收获时间而言，ASC通常需要在之后的时间使用。这就需要根据需要为这些细胞的储存和复苏制定合适的条件和程序。本方案描述了ASC的冷冻保存和解冻方法，且不会损害储存细胞的活力。
