清醒大鼠部分失血性复苏的实验模型

Experimental Model of Partially Recovered Blood Loss in Awake Rats.

作者信息

Ismailova A M, Severyukhina M S, Palikov V A, Borozdina N A, Semushina S G, Cholina A V, Khokhlova O N, Rzhevsky D I, Slascheva G A, Dyachenko I A, Murashev A N

机构信息

Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Russia.

Pushchino Branch of Russian Biotechnological University (BIOTECH University), Pushchino, Russia.

出版信息

Bull Exp Biol Med. 2025 Apr;178(6):778-781. doi: 10.1007/s10517-025-06416-9. Epub 2025 May 30.

DOI:10.1007/s10517-025-06416-9

PMID:40445544

Abstract

We propose a new model of blood loss in awake rats with volume loss up to 40% of total blood volume in the phase of hemorrhagic shock and partial blood replacement up to final blood loss up to 30% of total blood volume. During blood withdrawal, the rats showed manifestation of baroreflex, increased blood levels of glucose and lactate, and decreased concentration of oxyhemoglobin. Partial blood volume compensation was followed by recovery of hemodynamics the levels of glucose and oxyhemoglobin. Thus, taking into account the awake status of rats and compensatory mechanisms, our model of blood loss with a fixed volume of blood drawing and its partial compensation is a relevant model for studying the efficacy of blood substitutes.

摘要

我们提出了一种清醒大鼠失血模型，在失血性休克阶段失血量可达总血容量的40%，并进行部分血液置换，直至最终失血量达总血容量的30%。在抽血过程中，大鼠表现出压力反射、血糖和乳酸水平升高以及氧合血红蛋白浓度降低。部分血容量补偿后，血流动力学、葡萄糖和氧合血红蛋白水平恢复。因此，考虑到大鼠的清醒状态和代偿机制，我们这种固定抽血量及其部分补偿的失血模型是研究血液替代品疗效的相关模型。