Larose P, Gutkowska J, du Souich P, Ong H
J Immunoassay. 1985;6(3):207-25. doi: 10.1080/01971528508063030.
A new solid-phase radioimmunoassay (RIA) has been developed for measuring arginine-vasopressin (AVP) in urine. AVP is first extracted from urine by adsorption on Vycor glass powder and eluted with acetone-water (60:40). The mean recovery is 75.3 +/- 2.2% (n = 18). The organic extract is evaporated to dryness and reconstituted in the assay buffer. Aliquots of this extract are then incubated with 125I-AVP in polystyrene LKB tubes previously coated with the antiserum (1:50000) for 48 hours. The free radioactive fraction is removed by aspiration and the tubes are counted. Values correlate well with those obtained by liquid-phase RIA using dextran-charcoal. Urinary AVP concentrations in normal Sprague-Dawley rats and rats with varying degrees of hydration have been measured.
已开发出一种新的固相放射免疫测定法（RIA）用于测量尿液中的精氨酸加压素（AVP）。首先通过吸附在Vycor玻璃粉上从尿液中提取AVP，并用丙酮 - 水（60:40）洗脱。平均回收率为75.3±2.2%（n = 18）。将有机提取物蒸发至干并在测定缓冲液中重新配制。然后将该提取物的等分试样与预先用抗血清（1:50000）包被的聚苯乙烯LKB管中的125I - AVP孵育48小时。通过抽吸除去游离放射性部分并对管进行计数。其值与使用葡聚糖 - 活性炭的液相RIA所获得的值相关性良好。已测量了正常Sprague - Dawley大鼠和不同程度水合作用大鼠的尿AVP浓度。
