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循环肿瘤细胞的分离与鉴定

Isolation and Characterization of Circulating Tumor Cells.

作者信息

Klusa Daria, Cochonneau Denis, Ollivier Emilie, Chalopin Antoine, Heymann Dominique

机构信息

Nantes Université, Nantes, France.

Institut de Cancérologie de l'Ouest, Tumour Heterogeneity and Precision Medicine Laboratory, Saint-Herblain, France.

出版信息

Methods Mol Biol. 2025;2885:99-112. doi: 10.1007/978-1-0716-4306-8_6.

DOI:10.1007/978-1-0716-4306-8_6
PMID:40448758
Abstract

This chapter predominately describes a procedure for isolating circulating tumor cells (CTCs) from the blood of bone cancer patients and their subsequent characterization using flow cytometry. Additionally, descriptions of alternative methods for the isolation and characterization of CTCs are also provided.

摘要

本章主要描述了从骨癌患者血液中分离循环肿瘤细胞（CTC）的程序，以及随后使用流式细胞术对其进行表征的方法。此外，还提供了CTC分离和表征的替代方法的描述。

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Isolation and Characterization of Circulating Tumor Cells.循环肿瘤细胞的分离与鉴定
Methods Mol Biol. 2025;2885:99-112. doi: 10.1007/978-1-0716-4306-8_6.
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本文引用的文献

1
Dynamics of CXCR4 positive circulating tumor cells in prostate cancer patients during radiotherapy.在放疗期间前列腺癌患者 CXCR4 阳性循环肿瘤细胞的动力学。
Int J Cancer. 2023 Jun 15;152(12):2639-2654. doi: 10.1002/ijc.34457. Epub 2023 Mar 13.
2
Single-Cell Phenotypic and Molecular Characterization of Circulating Tumor Cells Isolated from Cryopreserved Peripheral Blood Mononuclear Cells of Patients with Lung Cancer and Sarcoma.从肺癌和肉瘤患者冷冻保存的外周血单个核细胞中分离的循环肿瘤细胞的单细胞表型和分子特征。
Clin Chem. 2022 May 18;68(5):691-701. doi: 10.1093/clinchem/hvac019.
3
Clinical significance of circulating tumor cells and cell-free DNA in pediatric rhabdomyosarcoma.
循环肿瘤细胞和游离 DNA 在儿科横纹肌肉瘤中的临床意义。
Mol Oncol. 2022 May;16(10):2071-2085. doi: 10.1002/1878-0261.13197. Epub 2022 Mar 8.
4
The Role of Dielectrophoresis for Cancer Diagnosis and Prognosis.介电电泳在癌症诊断和预后中的作用。
Cancers (Basel). 2021 Dec 31;14(1):198. doi: 10.3390/cancers14010198.
5
Targeting CD99 Compromises the Oncogenic Effects of the Chimera EWS-FLI1 by Inducing Reexpression of Zyxin and Inhibition of GLI1 Activity.靶向CD99通过诱导桩蛋白重新表达和抑制GLI1活性来削弱嵌合体EWS-FLI1的致癌作用。
Mol Cancer Ther. 2022 Jan;21(1):58-69. doi: 10.1158/1535-7163.MCT-21-0189. Epub 2021 Oct 19.
6
Using a combination of gangliosides and cell surface vimentin as surface biomarkers for isolating osteosarcoma cells in microfluidic devices.利用神经节苷脂和细胞表面波形蛋白的组合作为表面生物标志物，在微流控装置中分离骨肉瘤细胞。
J Bone Oncol. 2021 Mar 24;28:100357. doi: 10.1016/j.jbo.2021.100357. eCollection 2021 Jun.
7
Circulating Tumor Cells and Biomarker Modulation with Olaratumab Monotherapy Followed by Olaratumab plus Doxorubicin: Phase Ib Study in Patients with Soft-Tissue Sarcoma.单药奥拉单抗治疗后联合多柔比星治疗软组织肉瘤患者的循环肿瘤细胞和生物标志物调节：Ib 期研究。
Mol Cancer Ther. 2021 Jan;20(1):132-141. doi: 10.1158/1535-7163.MCT-20-0441. Epub 2020 Nov 11.
8
Pediatric sarcomas display a variable EpCAM expression in a histology-dependent manner.小儿肉瘤以组织学依赖的方式表现出可变的上皮细胞黏附分子（EpCAM）表达。
Transl Oncol. 2020 Nov;13(11):100846. doi: 10.1016/j.tranon.2020.100846. Epub 2020 Aug 14.
9
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Drug Discov Today. 2019 Mar;24(3):763-772. doi: 10.1016/j.drudis.2018.11.017. Epub 2018 Nov 26.
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J Bone Oncol. 2018 Jul 26;12:83-90. doi: 10.1016/j.jbo.2018.07.002. eCollection 2018 Sep.