Klusa Daria, Cochonneau Denis, Ollivier Emilie, Chalopin Antoine, Heymann Dominique
Nantes Université, Nantes, France.
Institut de Cancérologie de l'Ouest, Tumour Heterogeneity and Precision Medicine Laboratory, Saint-Herblain, France.
Methods Mol Biol. 2025;2885:99-112. doi: 10.1007/978-1-0716-4306-8_6.
This chapter predominately describes a procedure for isolating circulating tumor cells (CTCs) from the blood of bone cancer patients and their subsequent characterization using flow cytometry. Additionally, descriptions of alternative methods for the isolation and characterization of CTCs are also provided.
本章主要描述了从骨癌患者血液中分离循环肿瘤细胞(CTC)的程序,以及随后使用流式细胞术对其进行表征的方法。此外,还提供了CTC分离和表征的替代方法的描述。