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塞内加尔婴猴(Galago senegalensis)丘脑底核神经元的亚分类:一项使用主成分分析的定量高尔基研究。

Subclassification of neurons in the subthalamic nucleus of the lesser bushbaby (Galago senegalensis): a quantitative Golgi study using principal components analysis.

作者信息

Pearson J C, Norris J R, Phelps C H

出版信息

J Comp Neurol. 1985 Aug 15;238(3):323-39. doi: 10.1002/cne.902380307.

DOI:10.1002/cne.902380307
PMID:4044919
Abstract

The morphology of neurons in the subthalamic nucleus (STN) of the lesser bushbaby (Galago senegalensis) is described in coronal brain sections processed by Golgi- and Nissl-staining techniques. Quantitative and statistical methods are used to evaluate (1) soma size and shape, (2) dendritic field size, shape, and branch frequency, (3) the number of dendritic and somatic spines per neuron, and (4) neuron location within the STN. Principal components analysis of these variables suggests that three classes of neurons are present. Two of these classes are considered to be projection cells, referred to as elongate-fusiform and radiate neurons, respectively. Elongatefusiform neurons have somata and dendritic fields which are large in diameter, extremely fusiform in shape, and give rise to few appendages. Somata and dendritic fields of radiate neurons are smaller in diameter, more rounded in shape, and support more spines than the elongate-fusiform neurons. The third class of cells in Galago STN is tentatively identified as consisting of interneurons on the basis of small soma and dendritic field size, thin and varicose dendritic morphology, and the presence of multilobulated dendritic appendages.

摘要

运用高尔基染色法和尼氏染色技术处理塞内加尔婴猴(Galago senegalensis)的冠状脑切片,描述了其丘脑底核(STN)中神经元的形态。采用定量和统计方法评估:(1)胞体大小和形状;(2)树突野大小、形状和分支频率;(3)每个神经元的树突棘和体细胞棘数量;(4)神经元在丘脑底核内的位置。对这些变量进行主成分分析表明存在三类神经元。其中两类被认为是投射细胞,分别称为细长梭形神经元和辐射状神经元。细长梭形神经元的胞体和树突野直径较大,形状极呈梭形,且附属物较少。辐射状神经元的胞体和树突野直径较小,形状更圆,且比细长梭形神经元有更多的棘。基于胞体和树突野较小、树突形态细且有静脉曲张以及存在多叶状树突附属物,塞内加尔婴猴丘脑底核中的第三类细胞初步被确定为中间神经元。

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