诱导多能干细胞向特定神经元亚型的分化进程。

Progression of differentiation of iPSCs into specific subtypes of neurons.

作者信息

Wang Jingwen, Ji Ruijie, Zhang Lei, Cheng Xiang, Zhang Xinhua

机构信息

Department of Anatomy, Medical School, Co-innovation Center of Neurodegeneration, Nantong University, Nantong, China.

Department of Anatomy, Medical School, Co-innovation Center of Neurodegeneration, Nantong University, Nantong, China; Clinical trial Center, Yancheng Third People's Hospital, The Sixth Affiliated Hospital of Nantong University, Yancheng, 224002, China.

出版信息

Differentiation. 2025 May-Jun;143:100869. doi: 10.1016/j.diff.2025.100869. Epub 2025 May 22.

DOI:10.1016/j.diff.2025.100869

PMID:40449261

Abstract

Induced pluripotent stem cells (iPSCs), generated through somatic cell reprogramming, exhibit self-renewal capacity and multilineage differentiation potential. In recent years, iPSC-derived neurons have emerged as a significant platform for researching mechanisms and developing therapies for neurological diseases. This paper reviews the targeted differentiation strategies of iPSCs into dopaminergic neurons, motor neurons, cholinergic neurons and medium spinal neurons, providing detailed insights into the differentiation processes. Additionally, this paper discusses the challenges associated with the future application of iPSCs-derived neurons in the treatment of nervous system diseases are also discussed in this paper, aiming to provide references for the application of iPSCs in cellular therapies for neurodegenerative disorders.

摘要

通过体细胞重编程产生的诱导多能干细胞（iPSC）具有自我更新能力和多向分化潜能。近年来，iPSC衍生的神经元已成为研究神经疾病机制和开发治疗方法的重要平台。本文综述了iPSC向多巴胺能神经元、运动神经元、胆碱能神经元和中脊髓神经元的定向分化策略，详细介绍了分化过程。此外，本文还讨论了iPSC衍生神经元在未来神经系统疾病治疗应用中面临的挑战，旨在为iPSC在神经退行性疾病细胞治疗中的应用提供参考。