Frotscher M, Léránth C
J Comp Neurol. 1985 Sep 8;239(2):237-46. doi: 10.1002/cne.902390210.
The cholinergic innervation of the rat hippocampus proper and fascia dentata was investigated by using a monoclonal antibody against choline acetyltransferase (ChAT). At the light microscopic level, thin varicose ChAT-immunoreactive fibers were observed mainly in the vicinity of the pyramidal and granular layers where they formed a fine network around proximal dendrites of pyramidal and granule cells. In addition, many ChAT-immuno-reactive fibers were found in the hilar region and in stratum oriens, radiatum, and lacunosum-moleculare of all hippocampal sectors. Electron microscopic analysis revealed ChAT immunoreactivity in thin unmyelinated varicose axons and terminals which established synaptic contacts. Asymmetric contacts of ChAT-immunoreactive terminals were found on small spines in the dendritic layers of the hippocampus proper and in the molecular layer of the fascia dentata. Symmetric synaptic contacts were formed on the cell bodies of pyramidal and granule cells. Both symmetric and asymmetric synaptic contacts occurred on dendritic shafts. The analysis of serial thin sections, which allows identification of postsynaptic elements, suggests that pyramidal cells, granule cells, and nonpyramidal neurons of the hippocampus receive a cholinergic input.
利用抗胆碱乙酰转移酶（ChAT）的单克隆抗体，对大鼠海马本部和齿状回的胆碱能神经支配进行了研究。在光学显微镜水平上，观察到细的曲张状ChAT免疫反应纤维主要位于锥体细胞层和颗粒细胞层附近，在锥体细胞和颗粒细胞的近端树突周围形成精细网络。此外，在所有海马区的海马门区、原层、辐射层和分子层中发现了许多ChAT免疫反应纤维。电子显微镜分析显示，在形成突触联系的细无髓曲张轴突和终末中存在ChAT免疫反应性。在海马本部树突层的小棘突上以及齿状回分子层中发现了ChAT免疫反应性终末的不对称突触联系。在锥体细胞和颗粒细胞的胞体上形成了对称突触联系。在树突干上同时存在对称和不对称突触联系。对连续薄切片的分析（这有助于识别突触后成分）表明，海马的锥体细胞、颗粒细胞和非锥体细胞接受胆碱能输入。
