Baninajarian Homa, Tahmourespour Arezoo, Vedaei Amirhossein, Ghasemi Davood
Dental Research Center, Department of Pediatric Dentistry, Dental Research Institute, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Basic Medical Sciences, Faculty of Dentistry, Islamic Azad University, Isfahan (Khorasgan) BranchIsfahan, Iran.
Saudi Dent J. 2025 May 31;37(4-6):10. doi: 10.1007/s44445-025-00014-1.
Considering the increase in the prevalence of early childhood caries, the effect of various types of frequently consumed infant milk formulas on the development of Streptococcus mutans biofilm was examined. Three samples of cow's milk-based formula (Aptamil, Bebelac, Nan), two samples of soy-based formula (Biomil soy and Isomil soy), and three samples of formulas containing hydrolyzed proteins (Aptamil HA, Bebelac HA, Nan HA) were selected. After adding the formulas to the Streptococcus mutans (ATCC 35668) culture media, the degree of bacterial biofilm formation was explored by assessing the optical density (OD) of the Cristal Violet dye in the decolorizing solution using an ELISA reader device. The analysis of the data was conducted using independent sample T-tests, ANOVA, and subsequently Fisher's LSD test using SPSS 22 software. A statistically significant difference was observed in the average levels of biofilm formation of Streptococcus mutans for the three types of understudy formulas (P < 0.001). In the presence of cow's milk-based formulas, the mean amount of biofilm formation was significantly greater than that of the soy-based (P = 0.003) and protein hydrolysate (P < 0.0001) formulas. However, no significant statistical difference was detected between the soy-based and protein hydrolysate formulas (P = 0.42). Aptamil exhibited the highest amount of biofilm formation with a mean OD of 1.81 whereas Aptamil HA showed the lowest amount of biofilm formation (OD = 0.61). The level of biofilm formation by Streptococcus mutans when exposed to cow's milk-based formula was noted to be greater than that observed with the soy-based and protein hydrolysate formulas.
考虑到幼儿龋齿患病率的增加，研究了各类经常食用的婴儿配方奶粉对变形链球菌生物膜形成的影响。选取了三种以牛奶为基础的配方奶粉样品（爱他美、贝乐嘉、能恩）、两种以大豆为基础的配方奶粉样品（百慧妈妈旗下的Biomil soy和美赞臣旗下的Isomil soy）以及三种含水解蛋白的配方奶粉样品（爱他美HA、贝乐嘉HA、能恩HA）。将这些配方奶粉添加到变形链球菌（ATCC 35668）培养基中后，使用酶标仪通过评估脱色溶液中结晶紫染料的光密度（OD）来探究细菌生物膜的形成程度。使用独立样本T检验、方差分析，随后使用SPSS 22软件进行Fisher最小显著差异检验对数据进行分析。观察到三种研究中的配方奶粉在变形链球菌生物膜形成的平均水平上存在统计学显著差异（P < 0.001）。在以牛奶为基础的配方奶粉存在的情况下，生物膜形成的平均量显著高于以大豆为基础的配方奶粉（P = 0.003）和蛋白水解物配方奶粉（P < 0.0001）。然而，以大豆为基础的配方奶粉和蛋白水解物配方奶粉之间未检测到显著的统计学差异（P = 0.42）。爱他美表现出最高的生物膜形成量，平均OD为1.81，而爱他美HA表现出最低的生物膜形成量（OD = 0.61）。注意到变形链球菌在接触以牛奶为基础的配方奶粉时的生物膜形成水平高于以大豆为基础的配方奶粉和蛋白水解物配方奶粉。
