Ohno Hayao, Komachiya Yuzuha, Iino Yuichi
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Tokyo, Tokyo, Japan.
Department of Biological Sciences, School of Science, The University of Tokyo, Tokyo, Tokyo, Japan.
MicroPubl Biol. 2025 May 16;2025. doi: 10.17912/micropub.biology.001600. eCollection 2025.
Taste avoidance learning in is regulated by the calsyntenin/alcadein homolog CASY-1 , which transports the insulin receptor DAF-2c to the synaptic region. This transport involves binding of the CASY-1 intracellular domain to the kinesin-1 (KIF5) complex. However, a previous study showed that the intracellular domain of CASY-1 is dispensable for learning. To investigate how CASY-1 functions, we performed functional domain mapping of CASY-1 . Both the cadherin domains of CASY-1 and its binding to kinesin-1 are individually dispensable, while simultaneous loss of both abolished the CASY-1 function, suggesting that CASY-1 enables robust intracellular transport through physical interactions with multiple proteins.
线虫中的味觉回避学习由钙连蛋白/阿尔卡德蛋白同源物CASY-1调节，它将胰岛素受体DAF-2c转运到突触区域。这种转运涉及CASY-1细胞内结构域与驱动蛋白-1（KIF5）复合体的结合。然而，先前的一项研究表明，CASY-1的细胞内结构域对于学习是可有可无的。为了研究CASY-1的功能，我们对CASY-1进行了功能结构域定位。CASY-1的两个钙黏蛋白结构域及其与驱动蛋白-1的结合各自都是可有可无的，但两者同时缺失则会消除CASY-1的功能，这表明CASY-1通过与多种蛋白质的物理相互作用实现强大的细胞内转运。
