Lozano-Soria Ana, Piedra-Buena Diaz Ana, Lopez-Moya Federico, Valverde-Urrea Miguel, Zubcoff Jose J, Martinez-Perez Jose Emilio, Lopez-Cepero Javier, Lopez-Llorca Luis V
Marine Sciences and Applied Biology, University of Alicante, Alicante, Spain.
Entomology Area, Plant Protection Department, Canarian Institute for Agronomy Research, Valle de Guerra, San Cristóbal de La Laguna, Tenerife, Canary Islands, Spain.
PeerJ. 2025 May 29;13:e19414. doi: 10.7717/peerj.19414. eCollection 2025.
Fungal volatile organic compounds (VOCs) with biological activity produced by entomopathogenic fungi ( and ) isolated from banana fields (Canary Islands) and the nematophagous fungus , used in biocontrol of root-knot nematodes, repel the banana weevil (BW), (Germar, 1824) under laboratory conditions. BW is the main pest of banana ( spp. (Linnaeus, 1753)). Its cryptic behavior makes it difficult to manage. Repellent VOCs alter BW behavior and can thus be used in sustainable pest management strategies. We evaluated fungal VOCs styrene (C1), benzothiazole (C2), 1,3-dimethoxybenzene (C5) and 2-cyclohepten-1-one (C7) in Canary Islands banana fields naturally infested with BW. 1,3-dimethoxybenzene (C5) significantly reduced the attraction of BW adults to sordidin (BW aggregation pheromone) in banana fields. C5 was detected in the field using GC-MS. C1 and C2 had a mild repellent effect influenced by seasonal changes. C7 VOC did not repel BW in the field. Site and season affected VOCs repellence to BW. Climate may influence VOCs evaporation and therefore their repellent efficacy. VOCs modify BW spatial ecology under field conditions. The inverse distance weighted (IDW) interpolation technique showed changes in BW infestation patterns after application of VOCs in the field. In conclusion, VOCs from biocontrol fungi reduced BW attraction to its aggregation pheromone in banana fields. These responses to experimental BW repellents were influenced by weather and BW population size. BW repellents have potential to be used in "push-pull" strategies to manage BW sustainably in banana crops.
从香蕉园（加那利群岛）分离出的具有生物活性的昆虫病原真菌产生的真菌挥发性有机化合物（VOCs）以及用于防治根结线虫的食线虫真菌，在实验室条件下能够驱避香蕉象甲（BW，Germar，1824）。香蕉象甲是香蕉（ spp. （Linnaeus，1753））的主要害虫。其隐匿行为使其难以防治。驱避性VOCs会改变香蕉象甲的行为，因此可用于可持续害虫管理策略。我们在自然感染香蕉象甲的加那利群岛香蕉园中评估了真菌VOCs苯乙烯（C1）、苯并噻唑（C2）、1,3 - 二甲氧基苯（C5）和2 - 环庚烯 - 1 - 酮（C7）。1,3 - 二甲氧基苯（C5）显著降低了香蕉园中香蕉象甲成虫对索地定（香蕉象甲聚集信息素）的吸引力。使用气相色谱 - 质谱联用仪（GC - MS）在田间检测到了C5。C1和C2受季节变化影响具有轻微的驱避作用。C7 VOC在田间并未驱避香蕉象甲。地点和季节会影响VOCs对香蕉象甲的驱避效果。气候可能会影响VOCs的蒸发，进而影响其驱避功效。VOCs在田间条件下改变了香蕉象甲的空间生态。反距离加权（IDW）插值技术显示在田间施用VOCs后香蕉象甲侵染模式的变化。总之，生物防治真菌产生的VOCs降低了香蕉园中香蕉象甲对其聚集信息素的吸引力。这些对实验性香蕉象甲驱避剂的反应受天气和香蕉象甲种群数量的影响。香蕉象甲驱避剂有潜力用于“推 - 拉”策略，以在香蕉作物中可持续地管理香蕉象甲。
