Al-Mutar Hayder Abdul-Kareem
Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
Open Vet J. 2025 Apr;15(4):1593-1598. doi: 10.5455/OVJ.2025.v15.i4.10. Epub 2025 Apr 30.
Cattle have a high potential for meat and calf production due to their flexibility and high-quality reproduction. Bacterial illnesses, on the other hand, pose a hazard to optimal reproduction.
This study aimed to identify the bacterial type that colonized in the vagina during estrus, pregnancy, and post-partum.
Using normal culture testing, a study was carried out with the secretions of the vagina. Healthy pregnant cross-breed cattle from the farm in order to detect the vaginal microbes. The study included 44 crossbred cows who were 3, 6, and 9 months pregnant, as well as six of each regular cycle (proestrus, estrus, metestrus, and diestrus). Using a syringe and pipette, aseptic cervicovaginal mucus/discharge samples were obtained during pregnancy, non-pregnancy, and postpartum. Gram's staining and biochemical tests were used to identify the samples after they had been managed for culture isolation on blood agar and MacConkey agar, the time of the experiment is 1 year.
All 44 vaginal swabs (100%) from cattle with different physiopathological conditions contained bacteria. During pregnancy, in descending order, were , , , and . The results showed that the vagina of crossbred cattle with different physiological circumstances had a vast diversity of bacteria.
Consequently, it is concluded that the vaginal cavity of pregnant cows demonstrates bacterial isolate dynamics influenced by endocrine conditions, highlighting its importance in the reproductive physiopathology of crossbred cattle.
由于其适应性和高质量的繁殖能力，牛在肉类和犊牛生产方面具有很高的潜力。另一方面，细菌性疾病对最佳繁殖构成威胁。
本研究旨在确定在发情期、妊娠期和产后定植于阴道的细菌类型。
利用常规培养检测方法，对阴道分泌物进行了一项研究。选取来自农场的健康怀孕杂交牛以检测阴道微生物。该研究包括44头怀孕3个月、6个月和9个月的杂交母牛，以及处于每个正常周期（发情前期、发情期、发情后期和间情期）的母牛各6头。在怀孕期、非孕期和产后，使用注射器和移液管获取无菌宫颈阴道黏液/分泌物样本。在血琼脂和麦康凯琼脂上进行培养分离处理后，使用革兰氏染色和生化试验对样本进行鉴定，实验时间为1年。
来自不同生理病理状况牛的所有44份阴道拭子（100%）均含有细菌。在怀孕期间，按降序排列为 、 、 和 。结果表明，处于不同生理状况的杂交牛阴道内存在种类繁多的细菌。
因此，得出结论，怀孕母牛的阴道腔显示出受内分泌状况影响的细菌分离动态，突出了其在杂交牛生殖生理病理学中的重要性。
