Rahawy M A, Al-Mutar Hayder Abdul-Kareem
Department of Surgery and Theriogenology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.
Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Iraq.
Vet World. 2021 Aug;14(8):1995-2001. doi: 10.14202/vetworld.2021.1995-2001. Epub 2021 Aug 3.
The study investigated the genetic polymorphism of the kisspeptin gene and its relationship with litter size in Cyprus and Iraqi black goats.
Blood samples (n=124) were collected from the two goat breeds reared at the Agricultural Research-Ruminant Research Station Breeding Station, Baghdad, Iraq. Genomic DNA was isolated using a DNA extraction kit. Polymerase chain reaction (PCR) was used to amplify the gene. All PCR products were sequenced and samples were used for further analysis using NCBI-Blast online on the exon 1 (595 bp) region of the gene.
The results of this study revealed a significantly (<0.05) larger litter size of the Cyprus goat breed than in the Iraqi black goats in the first and second parity. Three (893G/C, 973C/A, and 979T/G) substitutions relative to the gene reference sequence (GenBank ID: J × 047312.1, KC989928.1) were identified. Only the mutation g893G>C was identified as a single nucleotide polymorphism (SNP) associated with litter size. Furthermore, the average alleles in 1 gene of both types of goats 0.567 and 0.3715 GG, were recorded. The genotyping at locus g893C>G was demonstrating domination of fecundity quality litter size, Both genotypes SNP of GC were classified at this marked region of gene.
The study concluded that the role of the 1 gene in fecundity, revealing the status of this gene as an indicator in the assisted of caprine breeding selection.
本研究调查了塞浦路斯山羊和伊拉克黑山羊中 kisspeptin 基因的遗传多态性及其与产仔数的关系。
从伊拉克巴格达农业研究反刍动物研究站繁殖站饲养的两个山羊品种中采集血样（n = 124）。使用 DNA 提取试剂盒分离基因组 DNA。采用聚合酶链反应（PCR）扩增该基因。所有 PCR 产物均进行测序，并使用 NCBI - Blast 在线工具对该基因外显子 1（595 bp）区域的样本进行进一步分析。
本研究结果显示，在第一胎和第二胎中，塞浦路斯山羊品种的产仔数显著（<0.05）多于伊拉克黑山羊。相对于该基因参考序列（GenBank ID：J×047312.1，KC989928.1），鉴定出三个（893G/C、973C/A 和 979T/G）替换。仅突变 g893G>C 被鉴定为与产仔数相关的单核苷酸多态性（SNP）。此外，记录了两种山羊 1 基因的平均等位基因频率，GG 分别为 0.567 和 0.3715。g893C>G 位点的基因分型表明繁殖力质量产仔数占主导地位，两种基因型 SNP（GC）均在该基因的这一标记区域分类。
该研究得出结论，1 基因在繁殖力中的作用，揭示了该基因作为山羊辅助育种选择指标的地位。
