Hanafiah Muhammad, Sutriana Amalia, Helmi Teuku Zahrial, Fajriah Ruhul, Mulyana Happy, Sabila Cut Nanda, Dewantara Nadhira
Laboratory of Parasitology and Coordinator of One Health Research and Veterinary Economics, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia.
Laboratory of Pharmacology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia.
Open Vet J. 2025 Apr;15(4):1702-1712. doi: 10.5455/OVJ.2025.v15.i4.22. Epub 2025 Apr 30.
Abortion refers to the premature expulsion of a fetus from a dam, typically following the death of the fetus in utero. If fetal death occurs within the first 1-2 months of gestation, it is often classified as "early embryonic death." Such early losses generally proceed without noticeable clinical signs in the dam, and the termination of pregnancy may go undetected. In 2 months of gestation, fetal death usually results in evident clinical signs, culminating in the expulsion of the fetus and the associated fetal membranes.
The objective of this research was to identify the causative agent of abortion in achenes cows using the rose bengal test (RBT), complement fixation test (CFT), ELISA, and card agglutination test (CATT). The identified causative agents were then mapped according to geographical distribution.
Seventy-four blood serum samples were collected from cattle at the UPTD IBI Saree and 30 samples were collected from the UPTD Lembah Seulawah, Lhut/Jaya, Lhut/Teunom, and Reuleut/Ulim. The blood serum samples were then subjected to a series of serological tests: RBT and CFT, to detect the presence of (an agent thought to induce abortion). Detection of the infectious bovine rhinotracheitis (IBR) causative agent Bovine Herpesvirus-1 was performed using ELISA, whereas the Toxoplasma seropositive test was performed using CATT.
The abortion seropositive examination of cows by RBT and CFT methods achieved the same result 22.1%, with 15.3% in males and 28.8% in females. The Toxoplasma serologic test by CATT revealed a prevalence rate of 1.92% (2/140). The IBR examination with ELISA revealed that 85% of the samples were seropositive, 1.92% were suspected, and 16.35% were seronegative. From this series of examinations, we detected infections of Brucellosis, IBR, and toxins in the samples. Therefore, the infective causative agents of abortion in cows reared in the UPTD IBI Saree and other regions in Aceh are sp, , and IBR. The geographical distribution of the data showing abortion causative agents in Acehnese cows are in the UPTD IBI Saree, Lembah Seulawah, Lhut/Jaya, Lhut/Teunom, and Reuleut/Ulim.
The Acehnese cows examined by RBT, CFT, ELISA, and CATT were positive at different prevalences. The test results showed that the causative agent of abortion in cattle was not only caused by but could be caused by other agents or could occur in the same cattle caused by more than one agent simultaneously in one cattle.
流产是指胎儿在母体内过早排出，通常是在子宫内胎儿死亡之后。如果胎儿死亡发生在妊娠的前1 - 2个月内，通常被归类为“早期胚胎死亡”。这种早期流产通常在母体中没有明显的临床症状，妊娠终止可能未被察觉。在妊娠2个月时，胎儿死亡通常会导致明显的临床症状，最终导致胎儿及相关胎膜排出。
本研究的目的是使用玫瑰红试验（RBT）、补体结合试验（CFT）、酶联免疫吸附测定（ELISA）和卡片凝集试验（CATT）来确定阿切牛流产的病原体。然后根据地理分布对已确定的病原体进行绘图。
从UPTD IBI Saree的牛身上采集了74份血清样本，并从UPTD Lembah Seulawah、Lhut/Jaya、Lhut/Teunom和Reuleut/Ulim采集了30份样本。然后对血清样本进行了一系列血清学检测：RBT和CFT，以检测（一种被认为可导致流产的病原体）的存在。使用ELISA检测传染性牛鼻气管炎（IBR）病原体牛疱疹病毒-1，而使用CATT进行弓形虫血清阳性检测。
通过RBT和CFT方法对奶牛进行流产血清阳性检测的结果相同，为22.1%，其中雄性为15.3%，雌性为28.8%。通过CATT进行的弓形虫血清学检测显示患病率为1.92%（2/140）。ELISA进行的IBR检测显示，85%的样本血清呈阳性，1.92%疑似阳性，16.35%血清呈阴性。从这一系列检测中，我们在样本中检测到了布鲁氏菌病、IBR和毒素感染。因此，在UPTD IBI Saree和亚齐其他地区饲养的奶牛中，流产的感染病原体为sp、和IBR。显示亚齐牛流产病原体的数据的地理分布在UPTD IBI Saree、Lembah Seulawah、Lhut/Jaya、Lhut/Teunom和Reuleut/Ulim。
通过RBT、CFT、ELISA和CATT检测的亚齐牛在不同患病率下呈阳性。检测结果表明，牛流产的病原体不仅由引起，还可能由其他病原体引起，或者在同一头牛中可能同时由多种病原体引起。
