Lima Amanda Cristielly Nunes De, Bittar Joely Ferreira Figueiredo, Neto Otaviano de Souza Pires, Santos Eliane Macedo Sobrinho, da Silva Priscilla Elias Ferreira, Santos Hércules Otacílio, de Souza Cintya Neves, Dos Santos Franciane Gabrielle, de Almeida Anna Christina
Institute of Agricultural Sciences, Federal University of Minas Gerais, Montes Claros, Minas Gerais, Brazil.
Department of Veterinary Medicine, University of Agriculture UNIUBE, Uberaba, Minas Gerais, Brazil.
Vet World. 2025 Apr;18(4):837-850. doi: 10.14202/vetworld.2025.837-850. Epub 2025 Apr 19.
Trypanosomiasis caused by is a significant cause of reproductive inefficiency and economic losses in cattle farming. While its impact on reproduction is known, the molecular mechanisms underlying these effects remain poorly understood. This study aimed to investigate the molecular effects of on reproductive parameters in cattle and evaluate epidemiological risk factors associated with trypanosomiasis in beef cattle in northern Minas Gerais, Brazil.
A dual approach combining bioinformatics and epidemiological analyses was employed. Proteins linked to were identified using the UniProt database, and protein interaction networks were constructed using the String V.12 platform. The epidemiological study involved serological diagnosis of trypanosomiasis through indirect immunofluorescence in 383 serum samples collected from 14 herds. Risk factors such as farming system, animal replacement frequency, vector presence, vaccination practices, and reproductive history were assessed through statistical analysis.
Bioinformatics analyses suggested that may exert molecular effects on bovine reproduction through the expression of toll-like receptor 2, nuclear factor kappa B (NF-κB), and nuclear receptor coactivator 7 proteins. The overall prevalence of was 6.79%, with no direct association found between infection and reproductive performance. However, 57.7% of seropositive cattle belonged to farms with lower pregnancy rates, and 96% were from farms reporting abortions in the past 12 months. The semi-intensive/intensive farming system and shared use of syringes and needles during vaccination were identified as significant risk factors for infection.
The study provides evidence of spread in northern Minas Gerais and highlights the need for improved control strategies, including vector management and proper sanitary practices. Bioinformatic analysis suggests that may influence reproductive outcomes through the NF-κB signaling pathway, warranting further experimental validation. Future studies should investigate the molecular mechanisms of in high-prevalence herds to refine disease management and mitigation strategies.
由[病原体名称未给出]引起的锥虫病是养牛业繁殖效率低下和经济损失的重要原因。虽然其对繁殖的影响已为人所知，但其影响背后的分子机制仍知之甚少。本研究旨在调查[病原体名称未给出]对牛繁殖参数的分子影响，并评估巴西米纳斯吉拉斯州北部肉牛锥虫病相关的流行病学风险因素。
采用生物信息学和流行病学分析相结合的双重方法。使用UniProt数据库鉴定与[病原体名称未给出]相关的蛋白质，并使用String V.12平台构建蛋白质相互作用网络。流行病学研究包括通过间接免疫荧光对从14个牛群采集的383份血清样本进行锥虫病血清学诊断。通过统计分析评估养殖系统、动物更换频率、媒介存在、疫苗接种做法和繁殖史等风险因素。
生物信息学分析表明，[病原体名称未给出]可能通过Toll样受体2、核因子κB（NF-κB）和核受体共激活因子7蛋白的表达对牛繁殖产生分子影响。[病原体名称未给出]的总体流行率为6.79%，未发现感染与繁殖性能之间存在直接关联。然而，57.7%的血清阳性牛来自妊娠率较低牛场，96%来自过去12个月报告有流产情况的牛场。半集约化/集约化养殖系统以及疫苗接种期间共用注射器和针头被确定为[病原体名称未给出]感染的重要风险因素。
该研究提供了[病原体名称未给出]在米纳斯吉拉斯州北部传播的证据，并强调需要改进控制策略，包括媒介管理和适当的卫生措施。生物信息学分析表明，[病原体名称未给出]可能通过NF-κB信号通路影响繁殖结果，需要进一步的实验验证。未来的研究应调查高流行牛群中[病原体名称未给出]的分子机制，以完善疾病管理和缓解策略。
