Baine W B
J Gen Microbiol. 1985 Jun;131(6):1383-91. doi: 10.1099/00221287-131-6-1383.
To examine one possible mechanism of damage to leucocytes and tissue cells in legionellosis, seven species of Legionella were examined for cytolytic activity and for elaboration of phospholipase C, an enzyme that can damage mammalian cell membranes. Cytolysis was assessed using erythrocytes in agar. Phospholipase C was assayed by release of p-nitrophenol from p-nitrophenylphosphorylcholine and of tritiated phosphorylcholine from L-alpha-dipalmitoyl-[choline-methyl-3H]phosphatidylcholine. L. pneumophila, L. bozemanii, L. micdadei, L. dumoffii, L. gormanii, L. longbeachae and L. jordanis all lysed dog red blood cells, which have a high ratio of membrane phosphatidylcholine to sphingomyelin. The same strains hydrolysed varying amounts of p-nitrophenylphosphorylcholine; L. bozemanii exhibited the greatest activity. L. pneumophila, L. bozemanii, L. dumoffii, L. longbeachae and L. jordanis, but not L. micdadei, released tritiated phosphorylcholine from labelled substrate. These results indicate that several species of Legionella possess cytolytic capability; exotoxins with activity may play a role.
为研究军团病中白细胞和组织细胞损伤的一种可能机制，对七种军团菌进行了细胞溶解活性检测以及磷脂酶C（一种可损伤哺乳动物细胞膜的酶）分泌情况的检测。使用琼脂中的红细胞评估细胞溶解情况。通过对硝基苯磷酸胆碱释放对硝基苯酚以及从L-α-二棕榈酰-[胆碱-甲基-³H]磷脂酰胆碱释放氚化磷酰胆碱来测定磷脂酶C。嗜肺军团菌、博兹曼军团菌、米克戴德军团菌、杜莫夫军团菌、戈尔曼军团菌、长滩军团菌和乔丹军团菌均能溶解狗的红细胞，狗红细胞的膜磷脂酰胆碱与鞘磷脂比例较高。相同菌株水解不同量的对硝基苯磷酸胆碱；博兹曼军团菌表现出最大活性。嗜肺军团菌、博兹曼军团菌、杜莫夫军团菌、长滩军团菌和乔丹军团菌（但米克戴德军团菌未出现此情况）能从标记底物中释放氚化磷酰胆碱。这些结果表明几种军团菌具有细胞溶解能力；具有活性的外毒素可能起作用。
