Hattori H, Kanfer J N
J Neurochem. 1985 Nov;45(5):1578-84. doi: 10.1111/j.1471-4159.1985.tb07229.x.
The phospholipase D of the rat brain synaptic membrane possesses the highest activity of this enzyme of any mammalian tissue examined. The synaptic phospholipase D activity is latent and barely detectable in the absence of 4 mM sodium oleate. Several other fatty acids were either less effective or ineffective as stimulators of activity compared to this monounsaturated fatty acid. The activity was decreased by hemicholinium-3, an inhibitor of choline uptake and slightly activated by neostigmine, an acetylcholinesterase inhibitor. Incubation of synaptosomes in the presence of sodium oleate and acetyl-coenzyme A resulted in the formation of a product chromatographing with acetylcholine. Acetylcholine formation was nearly undetectable in the absence of sodium oleate or acetyl-coenzyme A. These results implicate synaptosomal phospholipase D in releasing choline from phosphatidylcholine for acetylcholine formation.
大鼠脑突触膜中的磷脂酶D在所有已检测的哺乳动物组织中，该酶具有最高的活性。突触磷脂酶D的活性是潜在的，在没有4 mM油酸钠的情况下几乎检测不到。与这种单不饱和脂肪酸相比，其他几种脂肪酸作为活性刺激剂的效果要么较差，要么无效。半胱氨酸-3（一种胆碱摄取抑制剂）可降低该活性，而新斯的明（一种乙酰胆碱酯酶抑制剂）可使其活性略有激活。在油酸钠和乙酰辅酶A存在的情况下孵育突触体，会产生一种与乙酰胆碱具有相同色谱特征的产物。在没有油酸钠或乙酰辅酶A的情况下，几乎检测不到乙酰胆碱的形成。这些结果表明，突触体磷脂酶D参与从磷脂酰胆碱释放胆碱以形成乙酰胆碱的过程。
