Phillips W D, Lai K, Bennett M R
J Neurocytol. 1985 Apr;14(2):309-25. doi: 10.1007/BF01258455.
The size and distribution of acetylcholine receptor clusters (AChR-C) on normal and aneural developing muscle fibres of the chick wing were studied by labelling AChR with fluorescent conjugates of alpha-bungarotoxin (alpha-BGT). AChR-C of a size typical of initial synaptic contacts (5 micron long) were present at 7 days incubation, shortly after the appearance of nerves, and were grouped in bands corresponding to muscle nerve branches. A regular distribution of large (approximately equal to 5 micron) AChR-C separated by 100-200 micron had developed by 10-14 days in the slow-tonic anterior latissimus dorsi and ulnimetacarpalis dorsalis muscles. The role of motor innervation in the formation of AChR-C was assessed by removing the brachial neural tube at 2 days incubation in order to prevent nerves entering the wing. Neural-tube removal prevented the appearance of the large AChR-C normally associated with the early synaptic contacts. Small AChR-C (less than 2 micron long) appeared in aneural muscles, but these were not grouped into bands characteristic of the large AChR-C in normal muscles. The results suggest that the formation of junctional AChR-C is dependent on nerves.
通过用α-银环蛇毒素（α-BGT）的荧光偶联物标记乙酰胆碱受体（AChR），研究了鸡翼正常和无神经发育的肌纤维上AChR簇（AChR-C）的大小和分布。在孵化7天时，即神经出现后不久，出现了典型的初始突触接触大小（5微米长）的AChR-C，并聚集成与肌肉神经分支相对应的条带。在慢张力的背阔肌前肌和尺掌背侧肌中，到10-14天时，已形成了由100-200微米隔开的大（约等于5微米）AChR-C的规则分布。通过在孵化2天时移除臂神经管以防止神经进入翅膀，评估了运动神经支配在AChR-C形成中的作用。移除神经管可防止通常与早期突触接触相关的大AChR-C出现。小的AChR-C（长度小于2微米）出现在无神经的肌肉中，但这些并没有聚集成正常肌肉中大AChR-C特有的条带。结果表明，连接性AChR-C的形成依赖于神经。
