Becker W, Månsson J E
J Nutr. 1985 Oct;115(10):1248-58. doi: 10.1093/jn/115.10.1248.
Rats from an inbred Sprague-Dawley strain were fed purified diets with a low (0.3% of the total energy), normal (3%) or high (10%) content of essential fatty acids (EFA) for several generations. Thirty- to 34-d-old male rats of at least the sixth generation to be fed these diets were given a single intragastric dose of [1-14C]linoleic acid in olive oil, and the respiratory CO2, urine and feces were collected for 20 h. The 14C activity was determined in the respiratory CO2, urine and feces as well as in total lipids and lipid classes of the whole animal and in nine tissue groups. The content of total lipids and lipid classes was similar in all groups. The rats in the low EFA group retained significantly more radioactivity (51%) in the tissues than the rats fed the normal EFA (34%) or the high EFA (27%) diets. In all groups most of the radioactivity was found in the skeletal muscles, skin, liver and white fat, but the retention was greater in the low EFA group than in the other groups, except in the white and brown fat. In the carcass and most tissues, the proportion of the retained 14C activity recovered in the phospholipids increased with decreasing EFA level in the diet, whereas in the triglycerides the opposite was found. Expressed as percent of administered dose, the total retention of radioactivity in the phospholipids was 31, 13 and 8% in the low, normal and high EFA group, respectively, while the retention in the triglycerides was about the same in all groups (17-18%).
将近交系斯普拉格-道利大鼠用必需脂肪酸（EFA）含量低（占总能量的0.3%）、正常（3%）或高（10%）的纯化日粮喂养几代。用这些日粮喂养的至少第六代30至34日龄雄性大鼠，经胃内给予一剂橄榄油中的[1-¹⁴C]亚油酸，并收集20小时的呼吸二氧化碳、尿液和粪便。测定呼吸二氧化碳、尿液和粪便以及全动物和九个组织组的总脂质和脂质类别的¹⁴C活性。所有组的总脂质和脂质类含量相似。低EFA组大鼠组织中保留的放射性（51%）显著高于正常EFA组（34%）或高EFA组（27%）的大鼠。在所有组中，大部分放射性存在于骨骼肌、皮肤、肝脏和白色脂肪中，但低EFA组的保留率高于其他组，白色和棕色脂肪除外。在胴体和大多数组织中，磷脂中回收的保留¹⁴C活性比例随着日粮中EFA水平的降低而增加，而在甘油三酯中则相反。以给药剂量的百分比表示，低、正常和高EFA组磷脂中放射性的总保留率分别为31%、13%和8%，而甘油三酯中的保留率在所有组中大致相同（17-18%）。
