• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

超极化[1-C]丙酮酸核磁共振波谱揭示了微尺度多细胞球体中肿瘤能量代谢的转变。

Hyperpolarized [1-C]pyruvate NMR spectroscopy reveals transition of tumor energy metabolism in microscale multicellular spheroids.

作者信息

Takakusagi Yoichi, Takakusagi Kaori, Inoue Kaori, Saito Keita, Homma Yoshimi, Ichikawa Kazuhiro

机构信息

Quantum Life Spin Group, Institute for Quantum Life Science (iQLS), National Institutes for Quantum Science and Technology (QST), 4-9-1 Anagawa, Inage-Ku, Chiba, 263-8555, Japan.

Department of Molecular Imaging and Theranostics, Institute for Quantum Medical Science (iQMS), National Institutes for Quantum Science and Technology (QST), 4-9-1 Anagawa, Inage-Ku, Chiba, 263-8555, Japan.

出版信息

Sci Rep. 2025 Jun 2;15(1):19303. doi: 10.1038/s41598-025-03454-1.

DOI:10.1038/s41598-025-03454-1
PMID:40456829
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12130504/
Abstract

Hyperpolarized (HP) [1-C]pyruvate nuclear magnetic resonance (NMR) spectroscopy was employed to investigate tumor energy metabolism in microscale multicellular spheroids of a few hundred micrometers in diameter, serving as a model of early-phase tumorigenesis in vivo. A three-dimensional static culture of murine squamous cell carcinoma (SCCVII) cells formed uniform smaller multicellular spheroids (~ 150 μm in diameter), without hypoxic or necrotic cores, yet these spheroids exhibited resistance to anti-tumor drugs. HP [1-C]pyruvate NMR spectroscopy of SCCVII spheroids revealed an increased conversion of pyruvate to lactate compared to monolayer cultures, indicating enhanced aerobic glycolysis in the aggregated cells. Additionally, HP spectroscopy differentiated the degree of aerobic glycolysis in human prostate tumor spheroids-DU145 (~ 120 μm) and PC-3 (~ 230 μm)-as evidenced by the upregulation of genes associated with lactate production and cellular transport. The Lac/Pyr ratio among spheroids correlated with those observed in homogenate samples of corresponding tumors grown in mice. These findings suggest that HP [1-C]pyruvate NMR spectroscopy may serve as a metabolic biomarker for early-phase tumorigenesis in vivo.

摘要

采用超极化（HP）[1 - C]丙酮酸核磁共振（NMR）光谱技术，对直径几百微米的微尺度多细胞球体中的肿瘤能量代谢进行研究，该球体作为体内早期肿瘤发生的模型。小鼠鳞状细胞癌（SCCVII）细胞的三维静态培养形成了均匀的较小多细胞球体（直径约150μm），没有缺氧或坏死核心，但这些球体表现出对抗肿瘤药物的抗性。SCCVII球体的HP [1 - C]丙酮酸NMR光谱显示，与单层培养相比，丙酮酸向乳酸的转化增加，表明聚集细胞中的有氧糖酵解增强。此外，HP光谱区分了人前列腺肿瘤球体 - DU145（约120μm）和PC - 3（约230μm）中的有氧糖酵解程度，这由与乳酸产生和细胞转运相关的基因上调所证明。球体中的Lac/Pyr比值与在小鼠体内生长的相应肿瘤匀浆样品中观察到的比值相关。这些发现表明，HP [1 - C]丙酮酸NMR光谱可能作为体内早期肿瘤发生的代谢生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad5c/12130504/45e53a0c904d/41598_2025_3454_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad5c/12130504/75ec1c08991a/41598_2025_3454_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad5c/12130504/caa291b6ae6d/41598_2025_3454_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad5c/12130504/2f78b9b08cc5/41598_2025_3454_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad5c/12130504/1ac2bd2f828e/41598_2025_3454_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad5c/12130504/45e53a0c904d/41598_2025_3454_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad5c/12130504/75ec1c08991a/41598_2025_3454_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad5c/12130504/caa291b6ae6d/41598_2025_3454_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad5c/12130504/2f78b9b08cc5/41598_2025_3454_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad5c/12130504/1ac2bd2f828e/41598_2025_3454_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad5c/12130504/45e53a0c904d/41598_2025_3454_Fig5_HTML.jpg

相似文献

1
Hyperpolarized [1-C]pyruvate NMR spectroscopy reveals transition of tumor energy metabolism in microscale multicellular spheroids.超极化[1-C]丙酮酸核磁共振波谱揭示了微尺度多细胞球体中肿瘤能量代谢的转变。
Sci Rep. 2025 Jun 2;15(1):19303. doi: 10.1038/s41598-025-03454-1.
2
Glycolytic pyruvate regulates P-Glycoprotein expression in multicellular tumor spheroids via modulation of the intracellular redox state.糖酵解丙酮酸通过调节细胞内氧化还原状态调节多细胞肿瘤球体中的 P-糖蛋白表达。
J Cell Biochem. 2010 Feb 1;109(2):434-46. doi: 10.1002/jcb.22422.
3
13C-MR Spectroscopic Imaging with Hyperpolarized [1-13C]pyruvate Detects Early Response to Radiotherapy in SCC Tumors and HT-29 Tumors.利用超极化[1-13C]丙酮酸进行的13C磁共振波谱成像可检测鳞状细胞癌肿瘤和HT-29肿瘤对放疗的早期反应。
Clin Cancer Res. 2015 Nov 15;21(22):5073-81. doi: 10.1158/1078-0432.CCR-14-1717. Epub 2015 Feb 11.
4
Hyperpolarized [1-C]-Pyruvate Magnetic Resonance Spectroscopic Imaging of Prostate Cancer Predicts Efficacy of Targeting the Warburg Effect.基于 1-C-丙酮酸的高极化磁共振波谱成像预测针对瓦博格效应的前列腺癌疗效。
Clin Cancer Res. 2018 Jul 1;24(13):3137-3148. doi: 10.1158/1078-0432.CCR-17-1957. Epub 2018 Mar 29.
5
Hyperpolarized [1-13C] pyruvate MR spectroscopy detect altered glycolysis in the brain of a cognitively impaired mouse model fed high-fat diet.高极化 [1-13C] 丙酮酸磁共振波谱检测高脂饮食喂养的认知障碍小鼠模型脑内糖酵解改变。
Mol Brain. 2018 Dec 18;11(1):74. doi: 10.1186/s13041-018-0415-2.
6
Combining Hyperpolarized Real-Time Metabolic Imaging and NMR Spectroscopy To Identify Metabolic Biomarkers in Pancreatic Cancer.结合超极化实时代谢成像和 NMR 波谱技术鉴定胰腺癌的代谢生物标志物。
J Proteome Res. 2019 Jul 5;18(7):2826-2834. doi: 10.1021/acs.jproteome.9b00132. Epub 2019 Jun 4.
7
Targeted AKT Inhibition in Prostate Cancer Cells and Spheroids Reduces Aerobic Glycolysis and Generation of Hyperpolarized [1-C] Lactate.在前列腺癌细胞和球体中靶向 AKT 抑制可减少有氧糖酵解和 [1-C] 乳酸的超极化生成。
Mol Cancer Res. 2018 Mar;16(3):453-460. doi: 10.1158/1541-7786.MCR-17-0458. Epub 2018 Jan 12.
8
Hyperpolarized [1-C]pyruvate-to-[1-C]lactate conversion is rate-limited by monocarboxylate transporter-1 in the plasma membrane.在细胞膜中，[1-C]丙酮酸到[1-C]乳酸的转化受单羧酸转运蛋白-1 的限制。
Proc Natl Acad Sci U S A. 2020 Sep 8;117(36):22378-22389. doi: 10.1073/pnas.2003537117. Epub 2020 Aug 24.
9
Simultaneous steady-state and dynamic 13C NMR can differentiate alternative routes of pyruvate metabolism in living cancer cells.同步稳态和动态 13C NMR 可区分活癌细胞中丙酮酸代谢的替代途径。
J Biol Chem. 2014 Feb 28;289(9):6212-24. doi: 10.1074/jbc.M113.543637. Epub 2014 Jan 10.
10
Comparison of kinetic models for analysis of pyruvate-to-lactate exchange by hyperpolarized 13 C NMR.比较分析 13 C 磁共振氢极化丙酮酸向乳酸转化的动力学模型。
NMR Biomed. 2012 Nov;25(11):1286-94. doi: 10.1002/nbm.2801. Epub 2012 Mar 26.

本文引用的文献

1
Design of Nuclear Magnetic Resonance Molecular Probes for Hyperpolarized Bioimaging.用于极化生物成像的磁共振分子探针的设计。
Angew Chem Int Ed Engl. 2021 Jun 25;60(27):14779-14799. doi: 10.1002/anie.201915718. Epub 2021 Feb 2.
2
Imaging breast cancer using hyperpolarized carbon-13 MRI.使用 13C 极化 MRI 进行乳腺癌成像。
Proc Natl Acad Sci U S A. 2020 Jan 28;117(4):2092-2098. doi: 10.1073/pnas.1913841117. Epub 2020 Jan 21.
3
3D tumor spheroids as in vitro models to mimic in vivo human solid tumors resistance to therapeutic drugs.
3D 肿瘤球体作为体外模型模拟体内人类实体肿瘤对治疗药物的耐药性。
Biotechnol Bioeng. 2019 Jan;116(1):206-226. doi: 10.1002/bit.26845. Epub 2018 Oct 27.
4
Hyperpolarized [1-C]-Pyruvate Magnetic Resonance Spectroscopic Imaging of Prostate Cancer Predicts Efficacy of Targeting the Warburg Effect.基于 1-C-丙酮酸的高极化磁共振波谱成像预测针对瓦博格效应的前列腺癌疗效。
Clin Cancer Res. 2018 Jul 1;24(13):3137-3148. doi: 10.1158/1078-0432.CCR-17-1957. Epub 2018 Mar 29.
5
Targeted AKT Inhibition in Prostate Cancer Cells and Spheroids Reduces Aerobic Glycolysis and Generation of Hyperpolarized [1-C] Lactate.在前列腺癌细胞和球体中靶向 AKT 抑制可减少有氧糖酵解和 [1-C] 乳酸的超极化生成。
Mol Cancer Res. 2018 Mar;16(3):453-460. doi: 10.1158/1541-7786.MCR-17-0458. Epub 2018 Jan 12.
6
Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging.超极化13C代谢磁共振波谱与成像
J Vis Exp. 2016 Dec 30(118):54751. doi: 10.3791/54751.
7
Induction of hypoxia and necrosis in multicellular tumor spheroids is associated with resistance to chemotherapy treatment.多细胞肿瘤球体中缺氧和坏死的诱导与化疗耐药相关。
Oncotarget. 2017 Jan 3;8(1):1725-1736. doi: 10.18632/oncotarget.13857.
8
Imaging Tumor Metabolism to Assess Disease Progression and Treatment Response.成像肿瘤代谢以评估疾病进展和治疗反应。
Clin Cancer Res. 2016 Nov 1;22(21):5196-5203. doi: 10.1158/1078-0432.CCR-16-0159. Epub 2016 Sep 8.
9
Metabolic shift toward oxidative phosphorylation in docetaxel resistant prostate cancer cells.多西他赛耐药前列腺癌细胞向氧化磷酸化的代谢转变。
Oncotarget. 2016 Sep 20;7(38):61890-61904. doi: 10.18632/oncotarget.11301.
10
Potential Clinical Roles for Metabolic Imaging with Hyperpolarized [1-(13)C]Pyruvate.超极化[1-(13)C]丙酮酸代谢成像的潜在临床作用
Front Oncol. 2016 Mar 11;6:59. doi: 10.3389/fonc.2016.00059. eCollection 2016.