Luo Zhiheng, Li Xipeng, You Caifa, Fang Laiping, Yang Delong, Wu Yunxia, Fan Zhijin, Zeng Qin, Chen Xu, Ma Hua, Zhao Junhui, Sang Shuo, Yang Hongju, Wang Dong, Zheng Judun, Liao Yuhui
Institute for Engineering Medicine, Kunming Medical University, Kunming, 650500, China.
College of Physics and Optoelectronic Engineering Shenzhen University, Shenzhen, 518060, China.
Small Methods. 2025 Jun 2:e2500486. doi: 10.1002/smtd.202500486.
Precise diagnosis of atherosclerosis (AS) is a crucial strategy for reducing acute cardiovascular events. Herein, a macrophage membrane (MM)-camouflaged biomimetic vesicle loaded with near-infrared-II (NIR-II) aggregation-induced emission luminogens (AIEgens) (P4TT@MM) is proposed to achieve localized in vivo imaging of atherosclerosis. The AIEgens characteristics of P4TT@MM effectively compensate for the fluorescence intensity decrease caused by the intramolecular torsional charge transfer (TICT) effect, even in foam cell-rich lesions. Benefiting from the biomimetic camouflage of MM and its strong affinity for atherosclerotic plaques, P4TT@MM enables precise targeting of lesion sites, successfully achieving high-contrast imaging of atherosclerotic plaques in the carotid arteries and aortas of the AS mice model. Compared with free P4TT, the NIR-II fluorescence intensity of P4TT@MM is increased by 140% in carotid arteries and 152% in aortas, respectively. Moreover, the P4TT@MM demonstrates robust biocompatibility combined with significant clinical translation potential. This study may present a promising tool for the clinical diagnosis of AS.
动脉粥样硬化（AS）的精确诊断是减少急性心血管事件的关键策略。在此，我们提出了一种负载近红外-II（NIR-II）聚集诱导发光剂（AIEgens）的巨噬细胞膜（MM）伪装的仿生囊泡（P4TT@MM），以实现动脉粥样硬化的体内局部成像。即使在富含泡沫细胞的病变中，P4TT@MM的AIEgens特性也能有效补偿分子内扭转电荷转移（TICT）效应导致的荧光强度下降。受益于MM的仿生伪装及其对动脉粥样硬化斑块的强亲和力，P4TT@MM能够精确靶向病变部位，成功实现AS小鼠模型颈动脉和主动脉中动脉粥样硬化斑块的高对比度成像。与游离P4TT相比，P4TT@MM在颈动脉和主动脉中的NIR-II荧光强度分别提高了140%和152%。此外，P4TT@MM表现出强大的生物相容性以及显著的临床转化潜力。本研究可能为AS的临床诊断提供一种有前景的工具。
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